製品の概要

  • 製品名Anti-L Dopa antibody
  • 製品の詳細
    Rabbit polyclonal to L Dopa
  • 特異性Using a conjugate L-DOPA-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:
    CompoundCross-reactivity ratio (a)
    L-DOPA-G-BSA1
    Dopamine-G-BSA1/>50,000
    Noradrenalin-G-BSA1/>50,000
    Tyrosine-G-BSA1/>50,000
    (a): L-DOPA-G-BSA concentration/unconjugated or conjugated catecholamine concentration at half displacement (b): non-reduced conjugate G = glutaraldehyde, BSA = bovine serum albumin
  • アプリケーション適用あり: ELISA, IHC-Frmore details
  • 免疫原

    L-dihydroxyphenylalanine (L-DOPA)-Glutaraldehyde-Carriers

  • 特記事項


    The antiserum was tested using the free floating PAP technique on rat dopaminergic areas. The anti-conjugated L-DOPA antibodies gave a good staining between 1/2000-1/5000 dilution in these areas.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab6426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/2000 - 1/5000.
IHC-Fr 1/2000 - 1/5000.

ターゲット情報

  • 関連性L Dopa is an intermediate in dopamine biosynthesis. Clinically, L Dopa is used in the management of Parkinson's disease. It is used as a prodrug to increase dopamine levels since it is able to cross the blood-brain barrier whereas dopamine itself cannot. Once L Dopa has entered the central nervous system (CNS), it is metabolised to dopamine by aromatic L amino acid decarboxylase. This also occurs in the peripheral tissues, causing adverse effects and decreasing the available dopamine to the CNS, so it is standard practice to co administer a peripheral DOPA decarboxylase inhibitor and often a catechol-O-methyl transferase (COMT) inhibitor.
  • 別名
    • 3 4 dihydroxy L phenylalanine antibody
    • L dihydroxyphenylalanine antibody
    • Levodopa antibody

プロトコール

Anti-L Dopa antibody (ab6426) 使用論文

ab6426 has not yet been referenced specifically in any publications.

Product Wall


You are correct that to produce an antibody to a small molecule it needs to be conjugated to a larger protein to enhance the immune response. ab6426 will detect will detect DOPA in tissues. In the Primary antibody note on the online datasheet, it ...

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Thank you for your email and I understand your concern. Ab5979 was originated and characterized outside Abcam. The reason why L-dopa was conjugated to glutaraldehyde and BSA was to make the protein more immunogenic. However, this exact process is the i...

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Yes, this antibody can also recognize human DOPA.

Yes, anti-L-Dopa antibody can recognize L-Dopa in avian tissue.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"