製品の概要

  • 製品名L-Amino Acid Assay Kit
  • テスト
    100 x 1 assay
  • サンプルの種類
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • アッセイタイプQuantitative
  • 検出感度
    <= 40 µM
  • 全工程の試験時間
    0h 30m
  • 製品の概要

    Abcam's L-Amino Acid Assay Kit provides a convenient means for directly detecting L-amino acid in biological samples. There is no requirement for sample pretreatment or purification when using this kit. The L-amino acid(s) level can be quantified using fluorometric (at Ex/Em = 535/587 nm) or colorimetric (at ? = 570 nm) methods in 96-well plates.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    L-Amino acids are the most essential elements in biology. Accurately quantitating L-amino acids in body fluids or purified samples may provide valuable information for diagnostic or basic research studies.

  • アプリケーション適用あり: Functional Studiesmore details

法規制情報

製品の特性

  • 関連性Amino acids perform various important functions for the body and serve as the materials for the body's cells, hormones, and enzymes. Of the standard amino acids, all by glycine can exist in either of the two optical isomers, called D and L enantiomers, which are mirror images of each other. The L-amino acids represent the vast majority of amino acids found in proteins during translation in the ribosome, and are therefore the most essential element in biology and metabolic functions.

アプリケーション

Our Abpromise guarantee covers the use of ab65347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

L-Amino Acid Assay Kit 画像

  • Standard curve (fluorimetrical): mean of duplicates (+/-) with background subtracted

  • Standard curve (colorimetrical): mean of duplicates (+/-SD) with background readings subtracted

  • L-Amino Acid Standard Curve.

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L-Amino Acid Assay Kit (ab65347) 使用論文

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ab83374 (Branched Chain Amino Acid Assay Kit). We have not tested this kit with cell or tissue lysates yet but only with fluids like Cell culture supernatant, Urine, Serum, Plasma and can therefore not provide more than a general protocol below. Read More

Merci de nous avoir contactés.

Le laboratoire m'a confirmé que nous n'avons malheureusement pas de données expérimentales pour ces kits sur des lysats musculaires.

Désolé de ne pas pouvoir vou...

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I am afraid that this is proprietary information which cannot be disclosed.

We apologize for any inconvenience this may cause.

I general, werecommend using several doses of your serum sample to ensure the readings are within the standard curve range. In essence, the dose range selection depends on your expectation of the L-amino-acid concentration in your samples.

An...

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Thank you for your enquiry.

I am sorry to confirm we do not currently have specific data in terms of what reagents and at what concentrations would interfere with this assay. Theoretically chemicals at high concentrations and ionic detergent...

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Thank you for your phone call today. I have gotten in touch with the production laboratory, and I was told that the kit only detects free L-amino acids. I hope this is helpful but please let me know if you have further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"