製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to l Afadin
  • アプリケーション
    適用あり: WB, IHC-FrFl, IHC-Fr, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Dog, Human, Monkey
  • 免疫原

    Synthetic peptide conjugated to maleimide-activated KLH, corresponding to amino acids 1817-1829 of Rat I Afadin with N-terminal cysteine.

  • 特記事項
    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-FrFl
IHC-Fr
ICC/IF
  • 追加情報
    ICC/IF: 1/500 (tested using MDCK cells and cultured human HepG2 cells).
    IHC-Fr: 1/1000 (tested in mouse liver sections).
    WB: 1/2000. Detects a band of approximately 200 kDa (in rat brain extracts).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton.
    • 関連疾患
      Note=A chromosomal aberration involving MLLT4 is associated with acute leukemias. Translocation t(6;11)(q27;q23) with MLL/HRX. The result is a rogue activator protein.
    • 配列類似性
      Contains 1 dilute domain.
      Contains 1 FHA domain.
      Contains 1 PDZ (DHR) domain.
      Contains 2 Ras-associating domains.
    • ドメイン
      The PDZ/DHR domain interacts with the C-terminus of nectin and the Pro-rich N-terminus domain interacts with F-actin.
    • 細胞内局在
      Cell junction > adherens junction. Not found at cell-matrix AJs.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AF6 antibody
      • AFAD_HUMAN antibody
      • Afadin antibody
      • ALL1-fused gene from chromosome 6 protein antibody
      • Mllt4 antibody
      • Protein Af-6 antibody
      • Protein AF6 antibody
      see all

    画像

    • All lanes : Anti-l Afadin antibody (ab11337) at 1/1000 dilution

      Lane 1 : Mouse hippocampal lysates
      Lane 2 : Mouse hippocampal lysates
      Lane 3 : Mouse hippocampal lysates (boiled)
      Lane 4 : Mouse hippocampal lysates (boiled)

      Lysates/proteins at 40 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit at 1/2000 dilution
      Developed using the ECL technique

      Performed under non-reducing conditions.

      Observed band size : 200 kDa (why is the actual band size different from the predicted?)


      Exposure time : 10 minutes

      This image is courtesy of an anonymous abreview.

      See Abreview

    参考文献

    This product has been referenced in:
    • Wang XD  et al. Nectin-3 links CRHR1 signaling to stress-induced memory deficits and spine loss. Nat Neurosci 16:706-13 (2013). Read more (PubMed: 23644483) »
    • Fournier G  et al. Loss of AF6/afadin, a marker of poor outcome in breast cancer, induces cell migration, invasiveness and tumor growth. Oncogene 30:3862-74 (2011). Read more (PubMed: 21478912) »

    See all 4 Publications for this product

    レビューと Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
    Sample
    Monkey Cell (COS)
    Specification
    COS
    Permeabilization
    Yes - 0.1% Triton
    Fixative
    Paraformaldehyde
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    投稿 Jan 02 2014

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
    Sample
    Mouse Cell (NIH3T3)
    Specification
    NIH3T3
    Permeabilization
    Yes - 0.1% Triton
    Fixative
    Paraformaldehyde
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    投稿 Jan 02 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (NIH3T3)
    Specification
    NIH3T3
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
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    投稿 Mar 19 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Monkey Cell (COS cells)
    Specification
    COS cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Triton X100
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
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    投稿 Feb 11 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Monkey Cell lysate - whole cell (COS1)
    Loading amount
    30 µg
    Specification
    COS1
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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    投稿 Nov 15 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Embryonic lung)
    Loading amount
    30 µg
    Specification
    Embryonic lung
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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    投稿 Nov 12 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Loading amount
    30 µg
    Specification
    HEK293
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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    投稿 Nov 12 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Mouse hippocampal sections (coronal, 25 um))
    Specification
    Mouse hippocampal sections (coronal, 25 um)
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    投稿 Sep 15 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Mouse hippocampal lysates)
    Loading amount
    40 µg
    Specification
    Mouse hippocampal lysates
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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    投稿 Sep 15 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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