製品の概要

  • 製品名Anti-Kv4.2 antibody
    Kv4.2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Kv4.2
  • アプリケーション適用あり: IHC-P, IHC-Fr, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from the C terminal domain of human Kv4.2.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab66639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/1000 - 1/5000. Predicted molecular weight: 70 kDa.
ICC/IF 1/1000.

ターゲット情報

  • 機能Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.
  • 組織特異性Highly expressed throughout the brain. Expression is very low or absent in other tissues.
  • 配列類似性Belongs to the potassium channel family. D (Shal) (TC 1.A.1.2) subfamily. Kv4.2/KCND2 sub-subfamily.
  • ドメインThe segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • 翻訳後修飾Phosphorylated on serine and threonine residues.
  • 細胞内局在Cell membrane. Cell projection > dendrite. Detected in dendrites in cultured hippocampal neurons. Association with KCNIP2 probably enhances cell surface expression.
  • Information by UniProt
  • 参照データベース
  • 別名
    • KCD2 antibody
    • KCND 2 antibody
    • KCND2 antibody
    • KCND2_HUMAN antibody
    • KIAA1044 antibody
    • MGC119702 antibody
    • MGC119703 antibody
    • Potassium voltage gated channel Shal related subfamily member 2 antibody
    • Potassium voltage-gated channel subfamily D member 2 antibody
    • RK 5 antibody
    • RK5 antibody
    • Voltage gated potassium channel Kv4.2 antibody
    • Voltage gated potassium channel subunit Kv4.2 antibody
    • Voltage sensitive potassium channel antibody
    • Voltage-gated potassium channel subunit Kv4.2 antibody
    see all

Anti-Kv4.2 antibody 画像

  • ICC/IF image of ab66639 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66639, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Kv4.2 antibody (ab66639) 使用論文

ab66639 has not yet been referenced specifically in any publications.

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Thank you for your phone call. I hope that increasing the primary antibody concentration and adding a permeabilization step with Tween 20 will help to increase the observed signal. If not, please let me know and I will be happy to assist you further.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"