製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Kv4.2
  • アプリケーション
    適用あり: WB, ICC/IF, IP, ELISAmore details
  • 種交差性
    交差種: Rat
    交差が予測される動物種: Mouse, Rabbit, Human, Ferret
  • 免疫原

    Synthetic peptide:

    ASGPMPAPPRQERKRTQDALI

    conjugated to KLH, corresponding to amino acids 23-43 of Rat Kv4.2.

  • ポジティブ・コントロール
    • Transfected HEK293 cells containing Kv4.2 cDNA.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 70 kDa).
ICC/IF Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.
  • 組織特異性
    Highly expressed throughout the brain. Expression is very low or absent in other tissues.
  • 配列類似性
    Belongs to the potassium channel family. D (Shal) (TC 1.A.1.2) subfamily. Kv4.2/KCND2 sub-subfamily.
  • ドメイン
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • 翻訳後修飾
    Phosphorylated on serine and threonine residues.
  • 細胞内局在
    Cell membrane. Cell projection > dendrite. Detected in dendrites in cultured hippocampal neurons. Association with KCNIP2 probably enhances cell surface expression.
  • Information by UniProt
  • 参照データベース
  • 別名
    • KCD2 antibody
    • KCND 2 antibody
    • KCND2 antibody
    • KCND2_HUMAN antibody
    • KIAA1044 antibody
    • MGC119702 antibody
    • MGC119703 antibody
    • Potassium voltage gated channel Shal related subfamily member 2 antibody
    • Potassium voltage-gated channel subfamily D member 2 antibody
    • RK 5 antibody
    • RK5 antibody
    • Voltage gated potassium channel Kv4.2 antibody
    • Voltage gated potassium channel subunit Kv4.2 antibody
    • Voltage sensitive potassium channel antibody
    • Voltage-gated potassium channel subunit Kv4.2 antibody
    see all

Anti-Kv4.2 antibody 画像

  • ab16719 detecting Kv4.2 by ELISA. The sample was purified from rat neuronal cells and blocked with 1% milk for 1 hour at 18°C. The primary antibody was used at 1/1000 dilution, and incubated with sample for 1 hour at 18°C.       

    See Abreview

  • All lanes : Anti-Kv4.2 antibody (ab16719)

    Lane 1 : Lysate prepared from total cellular protein (intracellular + surface)
    Lane 2 : Lysate prepared from total cellular protein (intracellular + surface)
    Lane 3 : Lysate prepared from total cellular protein (intracellular + surface)
    Lane 4 : Lysate prepared from biotinylated, surface-expressed channels (as supported by the absence of the intracellular protein synaptophysin)
    Lane 5 : Lysate prepared from biotinylated, surface-expressed channels (as supported by the absence of the intracellular protein synaptophysin)
    Lane 6 : Lysate prepared from biotinylated, surface-expressed channels (as supported by the absence of the intracellular protein synaptophysin)


    Predicted band size : 70 kDa

    Image from Noam Y et al, J Biol Chem. 2010 May 7;285(19):14724-36. Epub 2010 Mar 9, Fig 8.

Anti-Kv4.2 antibody (ab16719) 使用論文

This product has been referenced in:
  • Yang CH  et al. A critical role for protein tyrosine phosphatase nonreceptor type 5 in determining individual susceptibility to develop stress-related cognitive and morphological changes. J Neurosci 32:7550-62 (2012). Read more (PubMed: 22649233) »
  • Noam Y  et al. Trafficking and surface expression of hyperpolarization-activated cyclic nucleotide-gated channels in hippocampal neurons. J Biol Chem 285:14724-36 (2010). WB ; Rat . Read more (PubMed: 20215108) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Rat Purified protein (neuronal)
Specification
neuronal
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 18°C
Type
Indirect
Username

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Verified customer

投稿 May 06 2009

Application
Western blot
Sample
Escherichia coli Purified protein (E.Coli)
Loading amount
0.05 µg
Specification
E.Coli
Gel Running Conditions
Reduced Denaturing (gradient 4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

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Verified customer

投稿 Jan 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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