Anti-Kv1.4 抗体 (ab16718)

製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Kv1.4
  • アプリケーション
    適用あり: ELISA, IP, ICC/IF, WBmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Cow
  • 免疫原

    Recombinant fragment:

    CNSHMPYGYAAQARARERERLAHSR

    , corresponding to amino acids 13-37 of Rat Kv1.4 (conjugated to KLH).

  • ポジティブ・コントロール
    • Rat hippocampal membranes and lysates of HEK293 cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.08% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    IgG fraction
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab16718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution.
IP Use a concentration of 1 - 10 µg/ml.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 73 kDa. Detects a band of approximately 76 KDa in HEK293 lysate and 90 kDa in rat hippocampal membranes.

ターゲット情報

  • 機能
    Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
  • 配列類似性
    Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.4/KCNA4 sub-subfamily.
  • ドメイン
    The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Voltage gated K+ channel HuKII antibody
    • Fetal skeletal muscle potassium channel antibody
    • HBK4 antibody
    • HK1 antibody
    • HPCN2 antibody
    • HUKII antibody
    • Kcna4 antibody
    • KCNA4_HUMAN antibody
    • KCNA4L antibody
    • KCNA8 antibody
    • kv1.4 antibody
    • PCN2 antibody
    • Potassium channel 2 antibody
    • Potassium voltage gated channel shaker related subfamily member 4 antibody
    • Potassium voltage gated channel subfamily A member 4 antibody
    • Potassium voltage-gated channel subfamily A member 4 antibody
    • Rapidly inactivating potassium channel antibody
    • Shaker related potassium channel Kv1.4 antibody
    • Type A potassium channel antibody
    • Voltage gated potassium channel HBK4 antibody
    • Voltage gated potassium channel HK1 antibody
    • Voltage gated potassium channel subunit Kv1.4 antibody
    • Voltage-gated K(+) channel HuKII antibody
    • Voltage-gated potassium channel HBK4 antibody
    • Voltage-gated potassium channel HK1 antibody
    • Voltage-gated potassium channel subunit Kv1.4 antibody
    see all

画像

  • Western blots using ab16718.

    1: HEK293 cells transfected with Kv1.4.

    2: Rat hippocampal membrane.

    The size difference observed can probably be explained by differential post-translational processing.

  • ab16718 detecting rat recombinant protein by ELISA. Kv1.4 amino terminal domain was immobilized on 96-well plate using streptavidin-biotin interaction. Wells were blocked in 1% milk for 30 minutes at 19°C. The primary antibody was diluted 1/500 & 1/750 in PBS-Tween(0.1%) and incubated for 1 hour at 19°C. HRP conjugated goat monoclonal to rabbit, 1/2000 dilution was used as secondary.

    See Abreview

参考文献

ab16718 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Rat Recombinant protein (E.Coli)
Specification
E.Coli
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 19°C
Type
Indirect
Username

Abcam user community

Verified customer

投稿 Nov 18 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Recombinant protein (E.Coli)
Loading amount
0.1 µg
Specification
E.Coli
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Aug 27 2008

Thank you for your enquiry. I apologize for the delay in finding out this information, I can now inform you that this antibody was raised against a peptide which was conjugated to KLH for immunization. I hope this information will be useful, please...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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