Anti-Kv1.1 potassium channel 抗体 (ab32433)

製品の概要

  • 製品名Anti-Kv1.1 potassium channel antibody
    Kv1.1 potassium channel 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Kv1.1 potassium channel
  • アプリケーション適用あり: ICC/IF, IHC-P, WB, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Rat Kv1.1.

    (Peptide available as ab32432.)

  • ポジティブ・コントロール
    • ab32433 tested positive in Western Blot using the following tissue lysates: Mouse brain and rat heart.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 21632505
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa).

ab32433 detects a band at 70kDa. While this differs to its predicted molecular weight of 56kDa, this migration has been observed in the literature (PMID: PMC2290329).

IHC-FoFr 1/3000.

ターゲット情報

  • 機能Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
  • 関連疾患Defects in KCNA1 are the cause of episodic ataxia type 1 (EA1) [MIM:160120]; also known as paroxysmal or episodic ataxia with myokymia (EAM) or paroxysmal ataxia with neuromyotonia. EA1 is an autosomal dominant disorder characterized by brief episodes of ataxia and dysarthria. Neurological examination during and between the attacks demonstrates spontaneous, repetitive discharges in the distal musculature (myokymia) that arise from peripheral nerve. Nystagmus is absent.
    Defects in KCNA1 are the cause of myokymia isolated type 1 (MK1) [MIM:160120]. Myokymia is a condition characterized by spontaneous involuntary contraction of muscle fiber groups that can be observed as vermiform movement of the overlying skin. Electromyography typically shows continuous motor unit activity with spontaneous oligo- and multiplet-discharges of high intraburst frequency (myokymic discharges). Isolated spontaneous muscle twitches occur in many persons and have no grave significance.
  • 配列類似性Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.1/KCNA1 sub-subfamily.
  • ドメインThe N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • 翻訳後修飾Palmitoylated on Cys-243; which may be required for membrane targeting.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AEMK antibody
    • EA1 antibody
    • Episodic ataxia with myokymia antibody
    • HBK1 antibody
    • HUK1 antibody
    • Kca1 1 antibody
    • Kcna1 antibody
    • KCNA1_HUMAN antibody
    • Kcpvd antibody
    • KV1.1 antibody
    • MBK1 antibody
    • mceph antibody
    • MGC124402 antibody
    • MGC126782 antibody
    • MGC138385 antibody
    • MK1 antibody
    • MK1, mouse, homolog of KV1.1 antibody
    • Potassium channel protein 1 antibody
    • Potassium voltage gated channel shaker related subfamily member 1 antibody
    • Potassium voltage gated channel subfamily A member 1 antibody
    • Potassium voltage gated channel, shaker related subfamily, member 1 (episodic ataxia with myokymia) antibody
    • Potassium voltage-gated channel subfamily A member 1 antibody
    • RBK1 antibody
    • RCK1 antibody
    • Shak antibody
    • Shaker related subfamily member 1 antibody
    • Voltage gated potassium channel subunit Kv1.1 antibody
    • Voltage-gated K(+) channel HuKI antibody
    • Voltage-gated potassium channel HBK1 antibody
    • Voltage-gated potassium channel subunit Kv1.1 antibody
    see all

Anti-Kv1.1 potassium channel antibody 画像

  • All lanes : Anti-Kv1.1 potassium channel antibody (ab32433) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa,23 kDa,45 kDa,55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

    ab32433 detects a band at 70kDa. While this differs to its predicted molecular weight of 56kDa, this migration has been observed in the literature (PMID: PMC2290329).

  • ICC/IF image of ab32433 stained human HEK 293 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab32433, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • IHC FoFR image of ab32433 stained sections of rat dorsal root ganglion (20 um). The tissues was from perfusion fixed animals with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.

    See Abreview

Anti-Kv1.1 potassium channel antibody (ab32433) 使用論文

This product has been referenced in:
  • Tomlinson SE  et al. Clinical, genetic, neurophysiological and functional study of new mutations in episodic ataxia type 1. J Neurol Neurosurg Psychiatry : (2013). Read more (PubMed: 23349320) »
  • Remke M  et al. Adult medulloblastoma comprises three major molecular variants. J Clin Oncol 29:2717-23 (2011). IHC-P ; Human . Read more (PubMed: 21632505) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Sample Mouse Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 22 2014

In Western Blot the following tissues lysates were used as positive control: Mouse brain, mouse testis, mouse heart, mouse skeletal muscle, rat liver, rat heart and rat kidney.
Slides of these tissues can therefore be used as positive control sinc...

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Thank you for contacting us.
The antibody ab32433 was validated in IHC-P using Human adult brain tumor tissue slides (medulloblastoma), see PubMed: 21632505 (http://www.ncbi.nlm.nih.gov/pubmed/21632505?dopt=Abstract) for more details.
I hope t...

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Thank you for contacting Abcam.

Below is the link to the dilution calculator that we discussed.

https://www.msu.edu/˜nixonjos/more/dilution.html?stock=1&needed=0.0001&volume=1&total=0.1

For you to get 10ml at 1ug/...

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Thank you for contacting us. Having checked our catalog for the appropriate products, I would like to send you the following recommendations. Where possible I looked for both monoclonal and polyclonal antibodies, so if not mentioned we unfortunately d...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Dorsal root ganglion)
Specification Dorsal root ganglion
Username

Dr. Sophie Pezet

Verified customer

投稿 Aug 03 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"