製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Ku80
  • アプリケーション
    適用あり: IHC-P, IPmore details
    適用なし: WB
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MVRSGNKAAV VLCMDVGFTM SNSIPGIESP FEQAKKVITM FVQRQVFAEN) of Human Ku80 (NP_066964.1).

  • ポジティブ・コントロール
    • HeLa cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab93863 was affinity purified using an epitope specific to Ku80 immobilized on solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab93863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 - 5 µg/mg of lysate.
  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 機能
      Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
    • 配列類似性
      Belongs to the ku80 family.
      Contains 1 Ku domain.
    • 発生段階
      Expression increases during promyelocyte differentiation.
    • ドメイン
      The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
    • 翻訳後修飾
      Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity.
      Sumoylated.
    • 細胞内局在
      Nucleus. Chromosome.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 86 kDa subunit of Ku antigen antibody
      • ATP dependent DNA helicase 2 subunit 2 antibody
      • ATP dependent DNA helicase II 80 kDa subunit antibody
      • ATP dependent DNA helicase II 86 Kd subunit antibody
      • ATP dependent DNA helicase II antibody
      • ATP-dependent DNA helicase 2 subunit 2 antibody
      • ATP-dependent DNA helicase II 80 kDa subunit antibody
      • CTC box binding factor 85 kDa antibody
      • CTC box-binding factor 85 kDa subunit antibody
      • CTC85 antibody
      • CTCBF antibody
      • DNA repair protein XRCC5 antibody
      • KARP 1 antibody
      • KARP1 antibody
      • Ku 80 antibody
      • Ku autoantigen 80kDa antibody
      • Ku80 antibody
      • Ku86 antibody
      • Ku86 autoantigen related protein 1 antibody
      • KUB 2 antibody
      • KUB2 antibody
      • Lupus Ku autoantigen protein p86 antibody
      • NFIV antibody
      • Nuclear factor IV antibody
      • Thyroid lupus autoantigen antibody
      • Thyroid-lupus autoantigen antibody
      • TLAA antibody
      • X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) antibody
      • X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody
      • X-ray repair cross-complementing protein 5 antibody
      • Xray repair complementing defective repair in Chinese hamster cells 5 antibody
      • XRCC 5 antibody
      • XRCC5 antibody
      • XRCC5_HUMAN antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Ku80 with ab93863 at 1/1000 (1µg/ml). Detection: DAB.
    • ab93863 was used to immunoprecipitate Ku80 at 3 µg/mg of HeLa whole cell lysate. 20% of the immunoprecipitate was loaded on the gel and probed with a different anti-Ku80 antibody at 0.4 µg/ml.
      Detection: chemiluminescence with an exposure time of 30 seconds.

    参考文献

    ab93863 has not yet been referenced specifically in any publications.

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