製品の概要

  • 製品名Anti-Ku80 antibody [9403]
    Ku80 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [9403] to Ku80
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Zebrafish
  • 免疫原

    Recombinant protein encoding an N terminalfragment of human Ku 80 expressed in E. coli.

  • ポジティブ・コントロール
    • HeLa, HepG2, T24, and SKOV3 whole cell lysates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPhosphate buffered saline
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)Prepared in PBS from tissue culture supernatant.
  • ポリ/モノモノクローナル
  • クローン名9403
  • ミエローマNS1
  • アイソタイプIgG
  • 軽鎖の種類unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB 1/2000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).

ターゲット情報

  • 機能Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
  • 配列類似性Belongs to the ku80 family.
    Contains 1 Ku domain.
  • 発生段階Expression increases during promyelocyte differentiation.
  • ドメインThe EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
  • 翻訳後修飾Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity.
    Sumoylated.
  • 細胞内局在Nucleus. Chromosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 86 kDa subunit of Ku antigen antibody
    • ATP dependent DNA helicase 2 subunit 2 antibody
    • ATP dependent DNA helicase II 80 kDa subunit antibody
    • ATP dependent DNA helicase II 86 Kd subunit antibody
    • ATP dependent DNA helicase II antibody
    • ATP-dependent DNA helicase 2 subunit 2 antibody
    • ATP-dependent DNA helicase II 80 kDa subunit antibody
    • CTC box binding factor 85 kDa antibody
    • CTC box-binding factor 85 kDa subunit antibody
    • CTC85 antibody
    • CTCBF antibody
    • DNA repair protein XRCC5 antibody
    • KARP 1 antibody
    • KARP1 antibody
    • Ku 80 antibody
    • Ku autoantigen 80kDa antibody
    • Ku80 antibody
    • Ku86 antibody
    • Ku86 autoantigen related protein 1 antibody
    • KUB 2 antibody
    • KUB2 antibody
    • Lupus Ku autoantigen protein p86 antibody
    • NFIV antibody
    • Nuclear factor IV antibody
    • Thyroid lupus autoantigen antibody
    • Thyroid-lupus autoantigen antibody
    • TLAA antibody
    • X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) antibody
    • X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody
    • X-ray repair cross-complementing protein 5 antibody
    • Xray repair complementing defective repair in Chinese hamster cells 5 antibody
    • XRCC 5 antibody
    • XRCC5 antibody
    • XRCC5_HUMAN antibody
    see all

Anti-Ku80 antibody [9403] 画像

  • ab3715 staining Ku80 in human primary fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 2% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/300 in PBS + 2% BSA) for 14 hours at 4°C. An Alexa Fluor® 594-conjugated goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview



  • Predicted band size : 80 kDa
    ab3715 at a 1/2000 dilution staining ~ 80 kDa Ku80 in 35µg of  T24 whole cell extract. ECL detection. ab3715 at a 1/2000 dilution staining ~ 80 kDa Ku80 in 35µg of T24 whole cell extract. ECL detection.


  • Predicted band size : 80 kDa

    Western blot using ab3715 at 1/500.

    Lane 1: HeLa Nuclear Extract
    Lane 2: HeLa Whole Cell Lysate
    Lane 3: A431 Whole Cell Lysate
    Lane 4: Jurkat Whole Cell Lysate
    Lane 5: HEK293 Whole Cell Lysate

    Secondary ab: Anti-mouse HRP (ab6728) (1/5000)
    Exposure time: 30 seconds.
    Lanes 1 to 10: 20µg of cell lysate per lane.
    Expected molecular weight: 80 kDa



     

    Western blot using ab3715 at 1/500.

    Lane 1: HeLa Nuclear Extract
    Lane 2: HeLa Whole Cell Lysate
    Lane 3: A431 Whole Cell Lysate
    Lane 4: Jurkat Whole Cell Lysate
    Lane 5: HEK293 Whole Cell Lysate

    Secondary ab: Rabbit polyclonal to Mouse IgG H&L (HRP) ab6728 (1/5000)
    Exposure time: 30 seconds.
    Lanes 1 to 10: 20µg of cell lysate per lane.
    Expected molecular weight: 80 kDa

Anti-Ku80 antibody [9403] (ab3715) 使用論文

This product has been referenced in:
  • Seluanov A  et al. Changes in the level and distribution of Ku proteins during cellular senescence. DNA Repair (Amst) 6:1740-8 (2007). Read more (PubMed: 17686666) »

See 1 Publication for this product

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Human Cell lysate - whole cell (HeLa cells)
Specification HeLa cells
Total protein in input 30 µg
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投稿 Jan 30 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Sample Human Cell (Human primary fibroblasts)
Specification Human primary fibroblasts
Permeabilization Yes - 0.2% Triton-X100
Fixative Paraformaldehyde
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投稿 Mar 03 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount 20 µg
Specification Mouse embryonic fibroblasts
Gel Running Conditions Reduced Denaturing (10% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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投稿 Mar 22 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 30 µg
Specification HeLa cells
Treatment Ku80 siRNA
Gel Running Conditions Reduced Denaturing (4-16% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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投稿 Mar 07 2012

Thank you for your enquiry and you patience in awaiting a response. The full immunogen sequence that this product recognises is deemed to be commercially sensitive by the originator, however I am able to provide the % similarities to certain key specie...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (lung and foreskin fibroblast cells)
Specification lung and foreskin fibroblast cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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投稿 Feb 16 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"