製品の概要

  • 製品名
    Anti-Ku80 antibody [5C5]
    Ku80 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5C5] to Ku80
  • アプリケーション
    適用あり: WB, ELISA, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 61-340 of Human Ku80

  • ポジティブ・コントロール
    • HeLa, MCF7, A549 and NIH3T3 cell lysates, Human tonsil and Human colon cancer tissues, Hela cells, recombinant Ku80 protein
  • 特記事項

    This product was changed from ascites to supernatant. Lot no’s high than GR133722-19 are from Tissue Culture Supernatant

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • バッファー
    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    Purified from tissue culture supernatant.
  • ポリ/モノ
    モノクローナル
  • クローン名
    5C5
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab119935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Detects a band of approximately 86 kDa (predicted molecular weight: 83 kDa).
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF 1/200 - 1/1000.

ターゲット情報

  • 機能
    Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
  • 配列類似性
    Belongs to the ku80 family.
    Contains 1 Ku domain.
  • 発生段階
    Expression increases during promyelocyte differentiation.
  • ドメイン
    The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
  • 翻訳後修飾
    Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity.
    Sumoylated.
  • 細胞内局在
    Nucleus. Chromosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 86 kDa subunit of Ku antigen antibody
    • ATP dependent DNA helicase 2 subunit 2 antibody
    • ATP dependent DNA helicase II 80 kDa subunit antibody
    • ATP dependent DNA helicase II 86 Kd subunit antibody
    • ATP dependent DNA helicase II antibody
    • ATP-dependent DNA helicase 2 subunit 2 antibody
    • ATP-dependent DNA helicase II 80 kDa subunit antibody
    • CTC box binding factor 85 kDa antibody
    • CTC box-binding factor 85 kDa subunit antibody
    • CTC85 antibody
    • CTCBF antibody
    • DNA repair protein XRCC5 antibody
    • KARP 1 antibody
    • KARP1 antibody
    • Ku 80 antibody
    • Ku autoantigen 80kDa antibody
    • Ku80 antibody
    • Ku86 antibody
    • Ku86 autoantigen related protein 1 antibody
    • KUB 2 antibody
    • KUB2 antibody
    • Lupus Ku autoantigen protein p86 antibody
    • NFIV antibody
    • Nuclear factor IV antibody
    • Thyroid lupus autoantigen antibody
    • Thyroid-lupus autoantigen antibody
    • TLAA antibody
    • X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) antibody
    • X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody
    • X-ray repair cross-complementing protein 5 antibody
    • Xray repair complementing defective repair in Chinese hamster cells 5 antibody
    • XRCC 5 antibody
    • XRCC5 antibody
    • XRCC5_HUMAN antibody
    see all

Anti-Ku80 antibody [5C5] 画像

  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissues using ab119935 at a dilution of 1/200 with DAB staining.
  • Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using ab119935 at a dilution of 1/200 with DAB staining.
  • Immunofluorescence analysis of Hela cells using ab119935 at a dilution of 1/200 (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Flow cytometric analysis of Hela cells using ab119935 at a dilution of 1/200 (green) and negative control (purple).
  • ELISA, using ab119935 at a dilution of 1/10000.
  • Anti-Ku80 antibody [5C5] (ab119935) at 1/1000 dilution + Human vascular smooth muscle cell at 20 µg with Milk at 5 %

    Secondary
    Goat anti-mouse polyclonal IRDye® 800CW at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 83 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

    Image is courtesy of an anonymous AbReview.

    See Abreview

  • All lanes : Anti-Ku80 antibody [5C5] (ab119935) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : NIH3T3 cell lysate


    Predicted band size : 83 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)

Anti-Ku80 antibody [5C5] (ab119935) 使用論文

This product has been referenced in:
  • Beck C  et al. PARP3 affects the relative contribution of homologous recombination and nonhomologous end-joining pathways. Nucleic Acids Res 42:5616-32 (2014). WB ; Human . Read more (PubMed: 24598253) »

See 1 Publication for this product

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (vascular smooth muscle cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
vascular smooth muscle cell
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 21°C
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投稿 Nov 04 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Na-citrate pH6
Sample
Mouse Tissue sections (Liver, P5)
Specification
Liver, P5
Permeabilization
Yes - Triton 0,05%
Fixative
Paraformaldehyde
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投稿 Sep 29 2014

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris gel)
Sample
Human Cell lysate - whole cell (MDA-MB-231 breast cancer cells)
Specification
MDA-MB-231 breast cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Aug 19 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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