製品の概要

  • 製品名Anti-Ku70 antibody [N3H10]
    Ku70 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [N3H10] to Ku70
  • アプリケーション適用あり: IHC-P, IP, WB, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human, Monkey
    交差が予測される動物種: Hamster, Xenopus laevis非交差種: Rabbit, Cow, Sea Urchin
  • 免疫原

    Human placental extract designated as PSE1-PL.

  • エピトープAmino acids 506 - 541.
  • ポジティブ・コントロール
    • BT474, HEP-G-2 or HeLa cells. Tonsil tissue sections.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
Flow Cyt Use at an assay dependent concentration.

ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
  • 配列類似性Belongs to the ku70 family.
    Contains 1 Ku domain.
    Contains 1 SAP domain.
  • 発生段階Expression does not increase during promyelocyte differentiation.
  • 翻訳後修飾Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.
  • 細胞内局在Nucleus. Chromosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5''-deoxyribose-5-phosphate lyase Ku70 antibody
    • 5''-dRP lyase Ku70 antibody
    • 70 kDa subunit of Ku antigen antibody
    • ATP dependent DNA helicase 2 subunit 1 antibody
    • ATP dependent DNA helicase II 70 kDa subunit antibody
    • ATP-dependent DNA helicase 2 subunit 1 antibody
    • ATP-dependent DNA helicase II 70 kDa subunit antibody
    • CTC box binding factor 75 kDa subunit antibody
    • CTC box-binding factor 75 kDa subunit antibody
    • CTC75 antibody
    • CTCBF antibody
    • DNA repair protein XRCC6 antibody
    • G22P1 antibody
    • Ku 70 antibody
    • Ku autoantigen p70 subunit antibody
    • Ku autoantigen, 70kDa antibody
    • Ku p70 antibody
    • Ku70 antibody
    • Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa antibody
    • Kup70 antibody
    • Lupus Ku autoantigen protein p70 antibody
    • ML8 antibody
    • Thyroid autoantigen 70kD (Ku antigen) antibody
    • Thyroid autoantigen antibody
    • Thyroid lupus autoantigen antibody
    • Thyroid lupus autoantigen p70 antibody
    • Thyroid-lupus autoantigen antibody
    • TLAA antibody
    • X ray repair complementing defective repair in Chinese hamster cells 6 antibody
    • X-ray repair complementing defective repair in Chinese hamster cells 6 antibody
    • X-ray repair cross-complementing protein 6 antibody
    • XRCC 6 antibody
    • Xrcc6 antibody
    • XRCC6_HUMAN antibody
    see all

Anti-Ku70 antibody [N3H10] 画像

  • ICC/IF image of ab3114 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3114, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Ab3114 staining human malignant breast carcinoma. Staining is localized to nuclear compartment.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Anti-Ku70 antibody [N3H10] (ab3114) at 1/500 dilution + whole cell lysate prepared from HeLa cells at 10 µg

    Secondary
    HRP conjugated goat anti-mouse IgG at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 70 kDa


    Exposure time : 1 minute

    Image courtesy of an anonymous Abreview.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab3114 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3114, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Ku70 antibody [N3H10] (ab3114) 使用論文

This product has been referenced in:
  • Mohni KN  et al. ATR pathway inhibition is synthetically lethal in cancer cells with ERCC1 deficiency. Cancer Res 74:2835-45 (2014). Read more (PubMed: 24662920) »
  • Yao H  et al. P21-PARP-1 pathway is involved in cigarette smoke-induced lung DNA damage and cellular senescence. PLoS One 8:e80007 (2013). WB ; Mouse . Read more (PubMed: 24244594) »

See all 9 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colon)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH9 LEICA EDTA 20 mins
Permeabilization No
Specification Colon
Blocking step (agent) for 5 minute(s) · Concentration: 10%
Fixative Formaldehyde
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Abcam user community

Verified customer

投稿 Feb 23 2016

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (7.5%)
Sample Mouse Tissue lysate - whole (lung homogenate)
Specification lung homogenate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Feb 27 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (HeLa)
Loading amount 20 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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投稿 Mar 26 2013

I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application us...

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Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cells - cervical epithelial cancer)
Specification HeLa cells - cervical epithelial cancer
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton/TBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Jan 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells - cervical epithelial cancer)
Loading amount 10 µg
Specification HeLa cells - cervical epithelial cancer
Gel Running Conditions Reduced Denaturing (4-20% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

投稿 Dec 09 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (25, 50 or 100 µg of U2OS whole cell extract)
Loading amount 25 µg
Specification 25, 50 or 100 µg of U2OS whole cell extract
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Apr 12 2011

Thank you for your enquiry. At Abcam we always interested to see our antibodies tested in new species. Given the antibodies that you have highlighted I consider your best option rabbit polyclonal to Ku70 (ab10878) as it is a polyclonal antibody and...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"