Anti-Ku70 抗体 - Carboxyterminal end (ab58470)


  • 製品名Anti-Ku70 antibody - Carboxyterminal end
    Ku70 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Ku70 - Carboxyterminal end
  • 特異性ab58470 detects endogenous levels of total Ku70 protein.
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from C terminal of human Ku70

  • ポジティブ・コントロール
    • Extracts from K562 cells, human breast carcinoma tissue




Our Abpromise guarantee covers the use of ab58470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 70 kDa.
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.


  • 機能Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
  • 配列類似性Belongs to the ku70 family.
    Contains 1 Ku domain.
    Contains 1 SAP domain.
  • 発生段階Expression does not increase during promyelocyte differentiation.
  • 翻訳後修飾Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.
  • 細胞内局在Nucleus. Chromosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5''-deoxyribose-5-phosphate lyase Ku70 antibody
    • 5''-dRP lyase Ku70 antibody
    • 70 kDa subunit of Ku antigen antibody
    • ATP dependent DNA helicase 2 subunit 1 antibody
    • ATP dependent DNA helicase II 70 kDa subunit antibody
    • ATP-dependent DNA helicase 2 subunit 1 antibody
    • ATP-dependent DNA helicase II 70 kDa subunit antibody
    • CTC box binding factor 75 kDa subunit antibody
    • CTC box-binding factor 75 kDa subunit antibody
    • CTC75 antibody
    • CTCBF antibody
    • DNA repair protein XRCC6 antibody
    • G22P1 antibody
    • Ku 70 antibody
    • Ku autoantigen p70 subunit antibody
    • Ku autoantigen, 70kDa antibody
    • Ku p70 antibody
    • Ku70 antibody
    • Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa antibody
    • Kup70 antibody
    • Lupus Ku autoantigen protein p70 antibody
    • ML8 antibody
    • Thyroid autoantigen 70kD (Ku antigen) antibody
    • Thyroid autoantigen antibody
    • Thyroid lupus autoantigen antibody
    • Thyroid lupus autoantigen p70 antibody
    • Thyroid-lupus autoantigen antibody
    • TLAA antibody
    • X ray repair complementing defective repair in Chinese hamster cells 6 antibody
    • X-ray repair complementing defective repair in Chinese hamster cells 6 antibody
    • X-ray repair cross-complementing protein 6 antibody
    • XRCC 6 antibody
    • Xrcc6 antibody
    • XRCC6_HUMAN antibody
    see all

Anti-Ku70 antibody - Carboxyterminal end 画像

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab58470 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated.
  • All lanes : Anti-Ku70 antibody - Carboxyterminal end (ab58470) at 1/500 dilution

    Lane 1 : extracts from K562 cells with no immunising peptide
    Lane 2 : extracts from K562 cells with immunising peptide

    Predicted band size : 70 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)

Anti-Ku70 antibody - Carboxyterminal end (ab58470) 使用論文

ab58470 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab58470.
Please use the links above to contact us or submit feedback about this product.