製品の概要

  • 製品名Anti-Ku70 antibody
    Ku70 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Ku70
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide from a region within amino acids 500-550 (PEQAVDLTLP KVEAMNKRLG SLVDEFKELV YPPDYNPEGK VTKRKHDNEG S) of Human Ku70 (NP_001460.1)

  • ポジティブ・コントロール
    • HeLa and 293T cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab93809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use at a concentration of 2-5 µg/mg lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 70 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
    • 配列類似性Belongs to the ku70 family.
      Contains 1 Ku domain.
      Contains 1 SAP domain.
    • 発生段階Expression does not increase during promyelocyte differentiation.
    • 翻訳後修飾Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.
    • 細胞内局在Nucleus. Chromosome.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 5''-deoxyribose-5-phosphate lyase Ku70 antibody
      • 5''-dRP lyase Ku70 antibody
      • 70 kDa subunit of Ku antigen antibody
      • ATP dependent DNA helicase 2 subunit 1 antibody
      • ATP dependent DNA helicase II 70 kDa subunit antibody
      • ATP-dependent DNA helicase 2 subunit 1 antibody
      • ATP-dependent DNA helicase II 70 kDa subunit antibody
      • CTC box binding factor 75 kDa subunit antibody
      • CTC box-binding factor 75 kDa subunit antibody
      • CTC75 antibody
      • CTCBF antibody
      • DNA repair protein XRCC6 antibody
      • G22P1 antibody
      • Ku 70 antibody
      • Ku autoantigen p70 subunit antibody
      • Ku autoantigen, 70kDa antibody
      • Ku p70 antibody
      • Ku70 antibody
      • Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa antibody
      • Kup70 antibody
      • Lupus Ku autoantigen protein p70 antibody
      • ML8 antibody
      • Thyroid autoantigen 70kD (Ku antigen) antibody
      • Thyroid autoantigen antibody
      • Thyroid lupus autoantigen antibody
      • Thyroid lupus autoantigen p70 antibody
      • Thyroid-lupus autoantigen antibody
      • TLAA antibody
      • X ray repair complementing defective repair in Chinese hamster cells 6 antibody
      • X-ray repair complementing defective repair in Chinese hamster cells 6 antibody
      • X-ray repair cross-complementing protein 6 antibody
      • XRCC 6 antibody
      • Xrcc6 antibody
      • XRCC6_HUMAN antibody
      see all

    Anti-Ku70 antibody 画像

    • HeLa whole cell lysate (1mg) was immunoprecipitated using ab93809 at 3µg/mg. 20% of the immunoprecipitate was used for Western blotting. ab93809 was used at 0.4 µg/ml and the resulting band analysed by chemiluminescence with an exposure time of 1 second.
    • All lanes : Anti-Ku70 antibody (ab93809) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 70 kDa


      Exposure time : 3 seconds

    Anti-Ku70 antibody (ab93809) 使用論文

    ab93809 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris gel)
    Sample Human Cell lysate - whole cell (MDA-MB-231 Breast cancer cells)
    Specification MDA-MB-231 Breast cancer cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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    投稿 Aug 19 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"