製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to KSR2
  • 特異性
    Detects endogenous levels of total KSR2 protein.
  • アプリケーション
    適用あり: WB, ELISA, ICC/IF, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide from an internal region of human KSR2

  • ポジティブ・コントロール
    • WB: 293 cell extract ICC/IF: HeLa cells

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab72753 was Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).
ELISA 1/40000.
ICC/IF 1/500 - 1/1000.
IP Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Location-regulated scaffold connecting MEK to RAF. Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.
  • 組織特異性
    Mainly expressed in brain and kidney.
  • 配列類似性
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • ドメイン
    The protein kinase domain is predicted to be catalytically inactive.
  • 翻訳後修飾
    Phosphorylated on Ser-474 by MARK3.
  • 細胞内局在
    Cytoplasm. Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ25965 antibody
    • hKSR 2 antibody
    • hKSR2 antibody
    • Kinase suppressor of Ras 2 antibody
    • KSR 2 antibody
    • Ksr2 antibody
    • KSR2_HUMAN antibody
    see all

画像

  • All lanes : Anti-KSR2 antibody (ab72753) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : 293 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 108 kDa
    Observed band size : 108 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of KSR2 in HeLa cells, using ab72753 at 1/500 dilution. Right panel was also treated with immunising peptide.
  • KSR2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Rabbit polyclonal to KSR2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab72753.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 108kDa; KSR2

参考文献

This product has been referenced in:
  • Castellanos-Martín A  et al. Unraveling heterogeneous susceptibility and the evolution of breast cancer using a systems biology approach. Genome Biol 16:40 (2015). Read more (PubMed: 25853295) »

See 1 Publication for this product

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