製品の概要
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製品名Anti-KSR2 antibody
KSR2 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to KSR2
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由来種Rabbit
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特異性Detects endogenous levels of total KSR2 protein.
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アプリケーション適用あり: WB, ELISA, ICC/IF, IPmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide from an internal region of human KSR2
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ポジティブ･コントロール
- WB: 293 cell extract ICC/IF: HeLa cells
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）ab72753 was Affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab72753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa). | |
| ELISA | 1/40000. | |
| ICC/IF | 1/500 - 1/1000. | |
| IP | Use a concentration of 5 µg/ml. |
ターゲット情報
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機能Location-regulated scaffold connecting MEK to RAF. Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.
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組織特異性Mainly expressed in brain and kidney.
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配列類似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
ドメインThe protein kinase domain is predicted to be catalytically inactive.
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翻訳後修飾Phosphorylated on Ser-474 by MARK3.
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細胞内局在Cytoplasm. Membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 283455 Human
- Entrez Gene: 333050 Mouse
- Omim: 610737 Human
- SwissProt: Q6VAB6 Human
- SwissProt: Q3UVC0 Mouse
- Unigene: 375836 Human
- Unigene: 437947 Mouse
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別名
- FLJ25965 antibody
- hKSR 2 antibody
- hKSR2 antibody
see all
画像
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Western blot - Anti-KSR2 antibody (ab72753)All lanes : Anti-KSR2 antibody (ab72753) at 1/500 dilution
Lane 1 : 293 cell extract
Lane 2 : 293 cell extract with immunising peptide at 10 µg
Lysates/proteins at 10 µg per lane.
Predicted band size: 108 kDa
Observed band size: 108 kDa
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-KSR2 antibody (ab72753)Immunofluorescence analysis of KSR2 in HeLa cells, using ab72753 at 1/500 dilution. Right panel was also treated with immunising peptide. -
Immunoprecipitation - Anti-KSR2 antibody (ab72753)KSR2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Rabbit polyclonal to KSR2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab72753.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 108kDa; KSR2
プロトコール
参考文献
This product has been referenced in:
- Castellanos-Martín A et al. Unraveling heterogeneous susceptibility and the evolution of breast cancer using a systems biology approach. Genome Biol 16:40 (2015). Read more (PubMed: 25853295) »
レビューと Q&A
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