製品の概要
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製品名Anti-KSR1 antibody [EPR2421Y]
KSR1 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [EPR2421Y] to KSR1
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アプリケーション適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC or IP -
種交差性交差種: Mouse, Rat, Human
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免疫原
A synthetic peptide corresponding to residues near the C-terminus of human KSR.
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ポジティブ･コントロール
- 293 cell lysate.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
精製度Protein A purified
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ポリ/モノモノクローナル
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クローン名EPR2421Y
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab68483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/500 - 1/2000. Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse and rat samples. See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol). |
ターゲット情報
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機能Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.
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配列類似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
翻訳後修飾Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated.
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細胞内局在Cytoplasm. Membrane. In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery.
- Information by UniProt
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参照データベース
- Entrez Gene: 8844 Human
- Entrez Gene: 16706 Mouse
- Entrez Gene: 360573 Rat
- Omim: 601132 Human
- SwissProt: Q8IVT5 Human
- SwissProt: Q61097 Mouse
- Unigene: 133534 Human
- Unigene: 4745 Mouse
see all -
別名
- BKSR 1 antibody
- BKSR1 antibody
- dKsr antibody
see all
Anti-KSR1 antibody [EPR2421Y] 画像
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![Flow Cytometry - Anti-KSR1 antibody [EPR2421Y] (ab68483)](http://a.abcam.com/ps/products/68/ab68483/Images/ab68483-272318-ab68483FC1.jpg)
Flow Cytometry - Anti-KSR1 antibody [EPR2421Y] (ab68483)Unpurified ab68483 staining KSR1 in NIH/3T3 (mouse embryo) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/230. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
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![Western blot - Anti-KSR1 antibody [EPR2421Y] (ab68483)](http://a.abcam.com/ps/products/68/ab68483/Images/ab68483-269814-ab68483KOWB.jpg)
Western blot - Anti-KSR1 antibody [EPR2421Y] (ab68483)
Predicted band size : 102 kDaLane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: KSR1 knockout HAP1 cell lysate (40 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HEK293 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab68483 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.Unpurified ab68483 was shown to recognize KSR1 when KSR1 knockout samples were used, along with additional cross-reactive bands. Wild-type and KSR1 knockout samples were subjected to SDS-PAGE. Ab68483 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KSR1 antibody [EPR2421Y] (ab68483)](http://a.abcam.com/ps/products/68/ab68483/Images/ab68483-286186-ab68483IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KSR1 antibody [EPR2421Y] (ab68483)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue sections labeling KSR1 with Purified ab68483 at 1:100 dilution (1.49 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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![Western blot - Anti-KSR1 antibody [EPR2421Y] (ab68483)](http://a.abcam.com/ps/products/68/ab68483/Images/ab68483-286066-ab68483WB3.jpg)
Western blot - Anti-KSR1 antibody [EPR2421Y] (ab68483)Anti-KSR1 antibody [EPR2421Y] (ab68483) at 1/1000 dilution (purified) + MEF (Mouse embryonic fibroblast (immortalized)) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size : 102 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)Blocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-KSR1 antibody [EPR2421Y] (ab68483)](http://a.abcam.com/ps/products/68/ab68483/Images/ab68483-286021-ab68483WB2.jpg)
Western blot - Anti-KSR1 antibody [EPR2421Y] (ab68483)Anti-KSR1 antibody [EPR2421Y] (ab68483) at 1/1000 dilution (purified) + 293 (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size : 102 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)Blocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-KSR1 antibody [EPR2421Y] (ab68483)](http://a.abcam.com/ps/products/68/ab68483/Images/ab68483-286020-ab68483WB1.jpg)
Western blot - Anti-KSR1 antibody [EPR2421Y] (ab68483)Anti-KSR1 antibody [EPR2421Y] (ab68483) at 1/1000 dilution (purified) + Rat-1 (Rat embryonic fibroblast) whole cell lysates
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size : 102 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)Blocking and diluting buffer: 5% NFDM/TBST
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![Western blot - KSR antibody [EPR2421Y] (ab68483)](http://a.abcam.com/ps/products/68/ab68483/Images/ab68483wb.gif)
Western blot - KSR antibody [EPR2421Y] (ab68483)Anti-KSR1 antibody [EPR2421Y] (ab68483) at 1/2000 dilution (unpurified) + 293 cell lysate at 10 µg
Secondary
goat anti rabbit HRP at 1/2000 dilution
Predicted band size : 102 kDa
Observed band size : 120 kDa (why is the actual band size different from the predicted?)
プロトコール
Anti-KSR1 antibody [EPR2421Y] (ab68483) 使用論文
ab68483 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"