製品の概要

  • 製品名Anti-KSR1 antibody
    KSR1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KSR1
  • 特異性This antibody detects endogenous levels of total KSR protein.
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A synthetic non-phosphopeptide derived from human KSR around the phosphorylation site of serine 392.

  • ポジティブ・コントロール
    • Human brain tissue and extracts from NIH/3T3 cells

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab52196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
IHC-P
  • 追加情報ELISA: 1/20000.
    IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    WB: 1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.
    • 配列類似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 protein kinase domain.
    • 翻訳後修飾Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated.
    • 細胞内局在Cytoplasm. Membrane. In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BKSR 1 antibody
      • BKSR1 antibody
      • dKsr antibody
      • EK 31 antibody
      • EK31 antibody
      • hb antibody
      • Kinase suppressor of Ras 1 antibody
      • Kinase suppressor of ras antibody
      • KSR 1 antibody
      • KSR antibody
      • KSR1 antibody
      • KSR1_HUMAN antibody
      • Positive Ras signaling mediator family member (86.4 kD) antibody
      • Positive Ras signaling mediator family member antibody
      • RSU 2 antibody
      • RSU2 antibody
      • SR 31 antibody
      • SR31 antibody
      • Suppressor of activated let 60 Ras SUR3 antibody
      • Suppressor of Ras1 31 antibody
      see all

    Anti-KSR1 antibody 画像

    • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab52196 at a 1/50 dilution.

      Left image un-treated.

      Right image treated with peptide.

    • All lanes : Anti-KSR1 antibody (ab52196) at 1/500 dilution

      Lane 1 : Extracts from NIH/3T3 cells treated with PDGF (50ng/ml, 20min).
      Lane 2 : Extracts from NIH/3T3 cells treated with PDGF (50ng/ml, 20min) and peptide.


      Predicted band size : 102 kDa
      Observed band size : 102 kDa

    Anti-KSR1 antibody (ab52196) 使用論文

    This product has been referenced in:
    • Turski ML  et al. A novel role for copper in Ras/mitogen-activated protein kinase signaling. Mol Cell Biol 32:1284-95 (2012). WB ; Mouse . Read more (PubMed: 22290441) »
    • Thompson T  et al. 1,25-dihydroxyvitamin D3 enhances the apoptotic activity of MDM2 antagonist nutlin-3a in acute myeloid leukemia cells expressing wild-type p53. Mol Cancer Ther 9:1158-68 (2010). WB ; Human . Read more (PubMed: 20406950) »

    See all 2 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab52196.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"