製品の概要

  • 製品名Anti-KRAS+HRAS+NRAS antibody
    KRAS+HRAS+NRAS 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KRAS+HRAS+NRAS
  • 特異性Immunogen sequence is 100% homologous to human KRAS, NRAS and HRAS.
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human, Zebrafish
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Saccharomyces cerevisiae, Drosophila melanogaster
  • 免疫原

    Synthetic peptide corresponding to Human KRAS+HRAS+NRAS aa 1-50 (N terminal).

  • ポジティブ・コントロール
    • Human fetal heart lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 22 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

Anti-KRAS+HRAS+NRAS antibody 画像



  • Predicted band size : 22 kDa
    Western blot analysis of Human heart tissue lysate labeling KRAS with ab84573 at 1µg/ml.


  • Predicted band size : 22 kDa
    Western blot analysis of 250ng Candida albicans Ras1P protein labeling KRAS with ab84573 at 1:5000. A HRP-conjugated anti-rabbit (1:10000) was used as the secondary antibody.
  • Anti-KRAS+HRAS+NRAS antibody (ab84573) at 1 µg/ml + Human fetal heart lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 22 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • ab84573 at 5 µg/ml staining KRAS in Human placenta by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab84573 at 5 µg/ml staining KRAS in Human placenta by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • All lanes : Anti-KRAS+HRAS+NRAS antibody (ab84573) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : Zebrafish heart homogenate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 22 kDa


    Exposure time : 30 seconds

Anti-KRAS+HRAS+NRAS antibody (ab84573) 使用論文

This product has been referenced in:
  • Yang K  et al. Effect of PLCe gene silencing on inhibiting the cancerous transformation of ulcerative colitis. Exp Ther Med 12:422-426 (2016). WB ; Mouse . Read more (PubMed: 27347072) »
  • Zhou C  et al. PTTG acts as a STAT3 target gene for colorectal cancer cell growth and motility. Oncogene 33:851-61 (2014). WB ; Human . Read more (PubMed: 23416975) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
Permeabilization Yes - Wash Buffer from Dako with Tween
Specification Liver
Fixative Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

投稿 Dec 28 2015

Thank you very much for your interest in ab84573.

Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

To our knowledge, ab84573 has not been tested in Mouse but it is predicted t...

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Merci pour ces précisions.

Comme convenu j'ai mis en place l'envoi d'une unité d'anti-KRAS ab55391. Le numéro de commande de remplacement gratuit de ce produit est *****. Vous recevrez prochainement un mail de confirmati...

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Thank you for contacting Abcam.

Under our Abpromise we guarantee that all of those antibodies you listed would work on human tissue in IHC-P. However I would recommend ab55391, as It has protocol information available on the website.
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Merci d'avoir pris le temps de remplir notre questionnaire et de nous avoir contactés. Je suis désolé d'apprendre que vous avez eu des difficultés à obtenir de bons résultats avec ab84573.

Notre poli...

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Je suis désolée d'apprendre que ab84573 ne vous donne pas de bons résultats en immunohistochimie.
Nous allons vous remplacer cet anticorps avec ab55391 si nous concluons aussi que cet anticorps ab84573. Nous avons eu pour l'instant aucun raison de...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human colon tumor cell- HCT116)
Loading amount 30 µg
Specification human colon tumor cell- HCT116
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Sep 14 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"