製品の概要

  • 製品名Anti-KPNA6 antibody
    KPNA6 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to KPNA6
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Cow, Dog, Pig, Cynomolgus Monkey, Orangutan
  • 免疫原

    Peptide with sequence

    C-STTGESVITREMVE

    , corresponding to internal sequence amino acids 74-87 of human KPNA6 (NP_036448.1).

  • ポジティブ・コントロール
    • Human skeletal muscle lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab82149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 60 kDa. A customer reported that milk can significantly suppress antigen-antibody interaction on nitrocellulose membrane in WB.
IHC-P Use a concentration of 3 - 6 µg/ml.

ターゲット情報

  • 機能Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
  • 組織特異性Widely expressed.
  • 配列類似性Belongs to the importin alpha family.
    Contains 10 ARM repeats.
    Contains 1 IBB domain.
  • Information by UniProt
  • 参照データベース
  • 製品の状態Cellular location: cytoplasm; nucleus.
  • 別名
    • FLJ11249 antibody
    • IMA7_HUMAN antibody
    • Importin alpha 7 subunit antibody
    • Importin alpha S2 antibody
    • Importin subunit alpha 7 antibody
    • Importin subunit alpha-7 antibody
    • IPOA 7 antibody
    • IPOA7 antibody
    • Karyopherin alpha 6 antibody
    • Karyopherin alpha 6 (importin alpha 7) antibody
    • Karyopherin subunit alpha 6 antibody
    • Karyopherin subunit alpha-6 antibody
    • KPNA 6 antibody
    • KPNA 7 antibody
    • Kpna6 antibody
    • KPNA7 antibody
    • MGC17918 antibody
    • OTTHUMP00000004532 antibody
    see all

Anti-KPNA6 antibody 画像

  • Anti-KPNA6 antibody (ab82149) at 0.1 µg/ml + human skeletal muscle lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 60 kDa
    Observed band size : 60 kDa


    Exposure time : 1 hour

    Some minor background is detected and is blocked by the immunizing peptide.
  • ab82149 at 3.8 µg/ml staining KPNA6 in Human adrenal gland tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-KPNA6 antibody (ab82149) 使用論文

ab82149 has not yet been referenced specifically in any publications.

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I am sending this email to inquire about the updates you might have about the WB analysis. There were few anti KPNA4 products that were proving difficult to use and as per your last email you were suppose to contact m...

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Two SRP1 antibodies failed, which is very strange. We have used SRP1 transfected 293T cells line in the characterization of antibody ab124646, this means that the 293T cells seems not expressing the protein. I would ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"