製品の概要

  • 製品名Anti-KPNA3 antibody
    KPNA3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KPNA3
  • アプリケーション適用あり: ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide, corresponding to a region within the N terminal amino acids 1-50 of Human KPNA3 (accession number: NP_002258.2)

  • ポジティブ・コントロール
    • Human anaplastic thyroid carcinoma, breast carcinoma, colon carcinoma, linitis plastica stomach cancer, ovarian carcinoma, prostate carcinoma, testicular seminoma.
  • 特記事項Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
IHC-P
  • 追加情報ICC/IF: 1/100.
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.

    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Recognizes NLSs of influenza A virus nucleoprotein probably through ARM repeats 7-9.
    • 組織特異性Ubiquitous. Highest levels in heart and skeletal muscle.
    • 配列類似性Belongs to the importin alpha family.
      Contains 10 ARM repeats.
      Contains 1 IBB domain.
    • ドメインConsists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
      The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
      The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
    • 細胞内局在Cytoplasm. Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • SRP1 gamma antibody
      • hSRP1 antibody
      • IMA4_HUMAN antibody
      • Importin alpha 3 antibody
      • Importin alpha 3 subunit antibody
      • Importin alpha 4 antibody
      • Importin alpha Q2 antibody
      • Importin subunit alpha-4 antibody
      • IPOA4 antibody
      • Karyopherin alpha 3 antibody
      • Karyopherin alpha 3 importin alpha 4 antibody
      • Karyopherin alpha 3 subunit antibody
      • Karyopherin subunit alpha 3 antibody
      • Karyopherin subunit alpha-3 antibody
      • KPNA 3 antibody
      • Kpna3 antibody
      • Qip2 antibody
      • SRP1 antibody
      • SRP1-gamma antibody
      • SRP1gamma antibody
      • SRP4 antibody
      see all

    Anti-KPNA3 antibody 画像

    • Detection of Human KPNA3 by Immunohistochemistry.
      Sample: FFPE section of Human ovarian carcinoma.
      Antibody: ab84706 used at a dilution of 1/250.
      Detection: DAB staining.
    • Detection of Human KPNA3 by Immunohistochemistry.
      Sample: FFPE section of Human testis.
      Antibody: ab84706 used at a dilution of 1/500.
      Detection: DAB staining.
    • Detection of Mouse KPNA3 by Immunohistochemistry.
      Sample: FFPE section of Mouse teratoma.
      Antibody: ab84706 used at a dilution of 1/250.
      Detection: DAB staining.
    • Detection of Human KPNA3.
      Sample: FFPE section of Human colon carcinoma.
      Antibody: ab84706 used at a dilution of 1/100.
      Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1/100.

    Anti-KPNA3 antibody (ab84706) 使用論文

    ab84706 has not yet been referenced specifically in any publications.

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