Anti-KPNA3 抗体 (ab84706)
Key features and details
- Rabbit polyclonal to KPNA3
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-KPNA3 antibody
KPNA3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KPNA3 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chimpanzee, Rhesus monkey -
免疫原
Synthetic peptide corresponding to Human KPNA3 aa 1-100 (N terminal).
Database link: IHC-00320 -
ポジティブ・コントロール
- IHC-P: Human testis, ovarian carcinoma. Mouse teratoma. ICC: Human colon carcinoma,
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特記事項
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84706の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | ||
IHC-P |
特記事項 |
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IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Recognizes NLSs of influenza A virus nucleoprotein probably through ARM repeats 7-9. -
組織特異性
Ubiquitous. Highest levels in heart and skeletal muscle. -
配列類似性
Belongs to the importin alpha family.
Contains 10 ARM repeats.
Contains 1 IBB domain. -
ドメイン
Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 3839 Human
- Entrez Gene: 16648 Mouse
- Entrez Gene: 706925 Rhesus monkey
- Omim: 601892 Human
- SwissProt: O00505 Human
- SwissProt: O35344 Mouse
- Unigene: 527919 Human
- Unigene: 25548 Mouse
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別名
- SRP1 gamma antibody
- hSRP1 antibody
- IMA4_HUMAN antibody
see all
画像
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Detection of Human KPNA3 by Immunohistochemistry. Sample: FFPE section of Human ovarian carcinoma. Antibody: ab84706 used at a dilution of 1/250.Detection: DAB staining.
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Detection of Human KPNA3 by Immunohistochemistry. Sample: FFPE section of Human testis. Antibody: ab84706 used at a dilution of 1/500.Detection: DAB staining.
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Detection of Mouse KPNA3 by Immunohistochemistry. Sample: FFPE section of Mouse teratoma. Antibody: ab84706 used at a dilution of 1/250.Detection: DAB staining.
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Detection of Human KPNA3. Sample: FFPE section of Human colon carcinoma. Antibody: ab84706 used at a dilution of 1/100.Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1/100.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab84706 は 1 報の論文で使用されています。
- Zhou H et al. miR-181b regulates vascular endothelial aging by modulating an MAP3K3 signaling pathway. FASEB J 36:e22353 (2022). PubMed: 35593587