製品の概要

  • 製品名Anti-KPNA2 antibody
    KPNA2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KPNA2
  • アプリケーション適用あり: IHC-P, WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1 and 50 of human KPNA2

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 60mM Citrate, 5mM PBS, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab70609 was affinity purified using an epitope specific to KPNA2 immobilized on solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab70609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/5000 - 1/15000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IP Use at 2-10 µg/mg of lysate.

ターゲット情報

  • 機能Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
  • 組織特異性Expressed ubiquitously.
  • 配列類似性Belongs to the importin alpha family.
    Contains 10 ARM repeats.
    Contains 1 IBB domain.
  • ドメインConsists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
    The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
    The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • IMA1_HUMAN antibody
    • Importin alpha 1 antibody
    • Importin alpha 2 antibody
    • Importin alpha 2 subunit antibody
    • Importin alpha P1 antibody
    • Importin subunit alpha-1 antibody
    • IPO A1 antibody
    • IPOA 1 antibody
    • IPOA1 antibody
    • Karyopherin alpha 2 antibody
    • Karyopherin alpha 2 RAG cohort 1 antibody
    • Karyopherin alpha 2 subunit antibody
    • Karyopherin subunit alpha-2 antibody
    • KPNA2 antibody
    • KPNA2 protein antibody
    • Pendulin antibody
    • QIP 2 antibody
    • QIP2 antibody
    • RAG cohort 1 antibody
    • RAG cohort protein 1 antibody
    • RCH 1 antibody
    • RCH1 antibody
    • SRP 1 antibody
    • SRP1 alpha antibody
    • SRP1 antibody
    • SRP1-alpha antibody
    • SRP1alpha antibody
    see all

Anti-KPNA2 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissues labelling KPNA2 with ab70609 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-KPNA2 antibody (ab70609) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 58 kDa
    Observed band size : 58 kDa


    Exposure time : 30 seconds

Anti-KPNA2 antibody (ab70609) 使用論文

ab70609 has not yet been referenced specifically in any publications.

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