製品の概要

製品の詳細

  • 由来Synthetic

特性

Our Abpromise guarantee covers the use of ab13744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Blocking - Blocking peptide for Anti-KMT6 / EZH2 antibody - ChIP Grade (ab3748)

  • 製品の状態Liquid
  • 備考

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

関連情報

  • 別名
    • Enhancer of zeste 2
    • enhancer of zeste 2 polycomb repressive complex 2 subunit
    • Enhancer of zeste homolog 2
    • Enhancer of zeste homolog 2 (Drosophila)
    • Enhancer of zeste, Drosophila, homolog 2
    • ENX 1
    • Enx 1h
    • ENX-1
    • ENX1
    • Enx1h
    • EZH 2
    • EZH1
    • EZH2
    • EZH2_HUMAN
    • EZH2b
    • Histone-lysine N-methyltransferase EZH2
    • KMT 6
    • KMT6
    • KMT6A
    • Lysine N-methyltransferase 6
    • MGC9169
    • WVS
    • WVS2
    see all
  • 機能Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes.
  • 組織特異性Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.
  • 配列類似性Belongs to the histone-lysine methyltransferase family. EZ subfamily.
    Contains 1 SET domain.
  • 発生段階Expression decreases during senescence of embryonic fibroblasts (HEFs). Expression peaks at the G1/S phase boundary.
  • 翻訳後修飾Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity.
  • 細胞内局在Nucleus.
  • Information by UniProt

KMT6 / EZH2 peptide (ab13744) 使用論文

ab13744 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Other

Review text: I used this product to do a dot blot specificity test for cross reactivity of modified histone antibodies and everything worked great. I made two dilutions of the peptide and spotted it on a nitrocellulose membrane using a Bio-Dot apparatus. Once the peptides were bound to the membrane I incubated with the appropriate primary and secondary antibodies at an assay dependent concentration and developed using ECL.
Sample: Human Purified protein

Primary antibody (in addition to 'EZH2 peptide')
Dilution: 1/5000

Secondary antibody
Name: Non-Abcam antibody was used: anti-rabbit
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/10000
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Verified customer

投稿 Oct 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"