Anti-KMT5A / SETD8 / Pr-SET7 抗体 (ab111691)
Key features and details
- Rabbit polyclonal to KMT5A / SETD8 / Pr-SET7
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-KMT5A / SETD8 / Pr-SET7 antibody
KMT5A / SETD8 / Pr-SET7 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KMT5A / SETD8 / Pr-SET7 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human KMT5A/ SETD8/ Pr-SET7 aa 50-300. Recombinant fragment, corresponding to a region within amino acids 81-290 of Human KMT5A/ SETD8/ Pr-SET7, isoform 1(42kda) and 2(39KDa)
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ポジティブ・コントロール
- NT2D1, IMR32, U-87 MG and MCF7 whole cell lysates; HeLa xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab111691の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
1/500 - 1/3000. Predicted molecular weight: 39 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 39 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. -
配列類似性
Belongs to the histone-lysine methyltransferase family. PR/SET subfamily.
Contains 1 SET domain. -
発生段階
Not detected during G1 phase. First detected during S through G2 phases, and peaks during mitosis (at protein level). -
ドメイン
Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity. -
細胞内局在
Nucleus. Chromosome. Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin. - Information by UniProt
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参照データベース
- Entrez Gene: 387893 Human
- Omim: 607240 Human
- SwissProt: Q9NQR1 Human
- Unigene: 443735 Human
- Unigene: 572262 Human
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別名
- H4 K20 HMTase antibody
- H4 K20 specific histone methyltransferase antibody
- H4-K20-HMTase SETD8 antibody
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画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab111691 は 3 報の論文で使用されています。
- Li J et al. LncRNA LINC00473 is involved in the progression of invasive pituitary adenoma by upregulating KMT5A via ceRNA-mediated miR-502-3p evasion. Cell Death Dis 12:580 (2021). PubMed: 34091587
- Wang K et al. Di-methylation of CD147-K234 Promotes the Progression of NSCLC by Enhancing Lactate Export. Cell Metab 33:160-173.e6 (2021). PubMed: 33406400
- Xu Y et al. MiRNA-340-5p mediates the functional and infiltrative promotion of tumor-infiltrating CD8+ T lymphocytes in human diffuse large B cell lymphoma. J Exp Clin Cancer Res 39:238 (2020). PubMed: 33168024