Anti-KMT3A / HYPB / HIF-1 抗体 (ab69836)

製品の概要

  • 製品名Anti-KMT3A / HYPB / HIF-1 antibody
    KMT3A / HYPB / HIF-1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KMT3A / HYPB / HIF-1
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Horse, Cat, Dog
  • 免疫原

    A region within synthetic peptide: ELNEEIKQSH SFSLQTPCSK GSELRMINKN PEREKAGSPA PSNRLNDSPT, corresponding to N terminal amino acids 577-626 of Human KMT3A/ HYPB/ HIF-1

  • ポジティブ・コントロール
    • HepG2 cell lysate. Human placenta.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab69836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately >70 kDa (predicted molecular weight: 288 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能Histone methyltransferase that methylates 'Lys-36' of histone H3. H3 'Lys-36' methylation represents a specific tag for epigenetic transcriptional activation. Probably plays a role in chromatin structure modulation during elongation via its interaction with hyperphosphorylated POLR2A. Binds DNA at promoters. May also act as a transcription activator that binds to promoters. Binds to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression.
  • 組織特異性Ubiquitously expressed.
  • 配列類似性Belongs to the histone-lysine methyltransferase family. SET2 subfamily.
    Contains 1 AWS domain.
    Contains 1 post-SET domain.
    Contains 1 SET domain.
    Contains 1 WW domain.
  • ドメインThe low charge region mediates the transcriptional activation activity.
  • 翻訳後修飾May be automethylated.
  • 細胞内局在Nucleus. Chromosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EC 2.1.1.43 antibody
    • FLJ16420 antibody
    • FLJ22472 antibody
    • FLJ23184 antibody
    • FLJ45883 antibody
    • HBP231 antibody
    • HIF 1 antibody
    • HIF-1 antibody
    • HIF1 antibody
    • HIP-1 antibody
    • Histone lysine N methyltransferase SETD2 antibody
    • Histone-lysine N-methyltransferase SETD2 antibody
    • hSET2 antibody
    • HSPC069 antibody
    • Huntingtin interacting protein 1 antibody
    • Huntingtin interacting protein antibody
    • Huntingtin interacting protein B antibody
    • Huntingtin interacting protein HYPB antibody
    • Huntingtin yeast partner B antibody
    • Huntingtin-binding protein, 231-KD antibody
    • Huntingtin-interacting protein 1 antibody
    • Huntingtin-interacting protein B antibody
    • HYPB antibody
    • KIAA1732 antibody
    • KMT3A antibody
    • Lysine N methyltransferase 3A antibody
    • Lysine N-methyltransferase 3A antibody
    • p231HBP antibody
    • SET domain containing 2 antibody
    • SET domain-containing protein 2 antibody
    • SET2 antibody
    • SETD2 antibody
    • SETD2_HUMAN antibody
    see all

Anti-KMT3A / HYPB / HIF-1 antibody 画像

  • All lanes : Anti-KMT3A / HYPB / HIF-1 antibody (ab69836) at 1.25 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Molecular weight marker

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 288 kDa
    Observed band size : >70 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded human placenta using ab69836 at 4 µg/ml. Positively labelled trophoblast cells are indicated with arrows.
  • ICC/IF image of ab69836 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69836, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-KMT3A / HYPB / HIF-1 antibody (ab69836) 使用論文

ab69836 has not yet been referenced specifically in any publications.

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Most antibodies will work fine with either BSA or milk.

When testing our antibodies, our lab uses 5% BSA as a blocking reagent, so I recommend this.

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