製品の概要

  • 製品名
    Anti-KMT2B / MLL4 antibody
    KMT2B / MLL4 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to KMT2B / MLL4
  • アプリケーション
    適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Pig, Monkey, Zebrafish, Gorilla, Common marmoset
  • 免疫原

    Synthetic peptide corresponding to Human KMT2B/ MLL4 aa 2703-2715 (C terminal). Entrez gene ID: 9757
    Sequence:

    CNCGAKRCRRFLN


    Database link: Q9UMN6

  • ポジティブ・コントロール
    • IHC-P: Human brain cortex and testis tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab104444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/128000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Histone methyltransferase. Methylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2. Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development. Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation.
  • 組織特異性
    Widely expressed. Highest levels in testis. Also found in brain, bone marrow, heart, muscle, kidney, placenta, spleen, thymus, prostate, ovary, intestine, colon, peripheral blood lymphocytes and pancreas. Often amplified in pancreatic carcinomas.
  • 配列類似性
    Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. TRX/MLL subfamily.
    Contains 3 A.T hook DNA-binding domains.
    Contains 1 CXXC-type zinc finger.
    Contains 1 FYR C-terminal domain.
    Contains 1 FYR N-terminal domain.
    Contains 3 PHD-type zinc fingers.
    Contains 1 post-SET domain.
    Contains 1 SET domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Histone-lysine N-methyltransferase 2B antibody
    • HRX2 antibody
    • KIAA0304 gene product antibody
    • KMT2B antibody
    • KMT2B_HUMAN antibody
    • Likely ortholog of mouse WW domain binding protein 7 antibody
    • Lysine N-methyltransferase 2B antibody
    • mixed lineage leukemia 4 antibody
    • Mixed lineage leukemia gene homolog 2 protein antibody
    • MLL2 antibody
    • myeloid lymphoid leukemia 4 antibody
    • Myeloid lymphoid or mixed lineage leukemia protein 4 antibody
    • Myeloid/lymphoid or mixed-lineage leukemia protein 4 antibody
    • Trithorax homolog 2 antibody
    • trithorax homologue 2 antibody
    • TRX2 antibody
    • WBP-7 antibody
    • WBP7 antibody
    • WW domain-binding protein 7 antibody
    see all

画像

  • ab104444, at 5 µg/ml, staining KMT2B / MLL4 in formalin-fixed, paraffin-embedded human brain cortex tissue by Immunohistochemistry. A biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen was used as the secondary antibody.

  • ab104444, at 5 µg/ml, staining KMT2B / MLL4 in formalin-fixed, paraffin-embedded human testis tissue by Immunohistochemistry. A biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen was used as the secondary antibody.

参考文献

This product has been referenced in:
  • Su CH  et al. Regulation of IL-20 Expression by Estradiol through KMT2B-Mediated Epigenetic Modification. PLoS One 11:e0166090 (2016). ChIP, IHC-P ; Human . Read more (PubMed: 27806114) »
  • Fulcoli FG  et al. Rebalancing gene haploinsufficiency in vivo by targeting chromatin. Nat Commun 7:11688 (2016). Read more (PubMed: 27256596) »

See all 2 Publications for this product

レビューと Q&A

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (5%)
Sample
Mouse Cell lysate - whole cell (primary bone marrow derived macrophages)
Specification
primary bone marrow derived macrophages
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Sep 10 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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