製品の概要

  • 製品名Anti-KMT2B / MLL4 antibody [4C10]
    KMT2B / MLL4 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4C10] to KMT2B / MLL4
  • アプリケーション適用あり: WB, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment (proprietary-tag) corresponding to Human KMT2B/ MLL4 aa 813-904. Entrez gene ID: 9757
    Sequence:

    KVAASMPLSPGGQMEEVAGAVKQISDRGPVRSEDESVEAKRERPSGPESP VQGPRIKHVCRHAAVALGQARAMVPEDVPRLSALPLRDRQDL


    Database link: Q9UMN6

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab56770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Histone methyltransferase. Methylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2. Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development. Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation.
  • 組織特異性Widely expressed. Highest levels in testis. Also found in brain, bone marrow, heart, muscle, kidney, placenta, spleen, thymus, prostate, ovary, intestine, colon, peripheral blood lymphocytes and pancreas. Often amplified in pancreatic carcinomas.
  • 配列類似性Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. TRX/MLL subfamily.
    Contains 3 A.T hook DNA-binding domains.
    Contains 1 CXXC-type zinc finger.
    Contains 1 FYR C-terminal domain.
    Contains 1 FYR N-terminal domain.
    Contains 3 PHD-type zinc fingers.
    Contains 1 post-SET domain.
    Contains 1 SET domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Histone-lysine N-methyltransferase 2B antibody
    • HRX2 antibody
    • KIAA0304 gene product antibody
    • KMT2B antibody
    • KMT2B_HUMAN antibody
    • Likely ortholog of mouse WW domain binding protein 7 antibody
    • Lysine N-methyltransferase 2B antibody
    • mixed lineage leukemia 4 antibody
    • Mixed lineage leukemia gene homolog 2 protein antibody
    • MLL2 antibody
    • myeloid lymphoid leukemia 4 antibody
    • Myeloid lymphoid or mixed lineage leukemia protein 4 antibody
    • Myeloid/lymphoid or mixed-lineage leukemia protein 4 antibody
    • Trithorax homolog 2 antibody
    • trithorax homologue 2 antibody
    • TRX2 antibody
    • WBP-7 antibody
    • WBP7 antibody
    • WW domain-binding protein 7 antibody
    see all

Anti-KMT2B / MLL4 antibody [4C10] 画像

  • Western blot against tagged recombinant protein immunogen using ab56770 MLL4 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa
  • Overlay histogram showing HL60 cells stained with ab56770 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56770, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-KMT2B / MLL4 antibody [4C10] (ab56770) 使用論文

ab56770 has not yet been referenced specifically in any publications.

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