Anti-KMT1E / SETDB1 抗体 - N-terminal (ab71194)


  • 製品名Anti-KMT1E / SETDB1 antibody - N-terminal
    KMT1E / SETDB1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KMT1E / SETDB1 - N-terminal
  • アプリケーション適用あり: IHC-P, ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (Human) conjugated to KLH

  • ポジティブ・コントロール
    • Jurkat tissue lysate.




Our Abpromise guarantee covers the use of ab71194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/10 - 1/50.
ELISA 1/1000.
WB 1/100 - 1/500. Predicted molecular weight: 143 kDa.


  • 機能Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.
  • 組織特異性Widely expressed. High expression in testis.
  • 配列類似性Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
    Contains 1 MBD (methyl-CpG-binding) domain.
    Contains 1 post-SET domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
    Contains 2 Tudor domains.
  • ドメインThe pre-SET, SET and post-SET domains are all required for methyltransferase activity. The 347-amino-acid insertion in the SET domain has no effect on the catalytic activity.
    Isoform 2 lacks all domains required for histone methyltransferase activity.
  • 細胞内局在Nucleus. Chromosome. Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AU022152 antibody
    • EC antibody
    • ERG-associated protein with SET domain antibody
    • ESET antibody
    • H3 K9 HMTase 4 antibody
    • H3-K9-HMTase 4 antibody
    • H3-K9-HMTase4 antibody
    • Histone H3 K9 methyltransferase 4 antibody
    • Histone H3-K9 methyltransferase 4 antibody
    • Histone-lysine N-methyltransferase SETDB1 antibody
    • KG1T antibody
    • KIAA0067 antibody
    • KMT1E antibody
    • Lysine N-methyltransferase 1E antibody
    • MGC90670 antibody
    • mKIAA0067 antibody
    • SET domain bifurcated 1 antibody
    • Set domain protein, bifurcated, 1 antibody
    • SETB1_HUMAN antibody
    • Setdb1 antibody
    see all

Anti-KMT1E / SETDB1 antibody - N-terminal 画像

  • All lanes : Anti-KMT1E / SETDB1 antibody - N-terminal (ab71194) at 1/500 dilution

    Lane 1 : Cell lysates prepared from non trasfected 293 cells
    Lane 2 : Cell lysates prepared from 293 cells transfected with SETDB1 gene

    Predicted band size : 143 kDa

Anti-KMT1E / SETDB1 antibody - N-terminal (ab71194) 使用論文

ab71194 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP on chip
Sample Mouse Cell lysate - nuclear (mouse ES cells)
Specification mouse ES cells
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Detection step Microarray

Dr. Steve Bilodeau

Verified customer

投稿 Oct 21 2009