Anti-KMT1E / SETDB1 抗体 - ChIP Grade (ab12317)

製品の概要

  • 製品名
    Anti-KMT1E / SETDB1 antibody - ChIP Grade
    KMT1E / SETDB1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KMT1E / SETDB1 - ChIP Grade
  • アプリケーション
    適用あり: ChIP, ICC/IF, WB, IHC-P, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Immunogen was a synthetic peptide, which represented a portion of human SET domain, bifurcated 1 encoded within exon 19 (LocusLink ID 9869).

  • ポジティブ・コントロール
    • 293T cells.
  • 特記事項
    Centrifuge tube to remove product from lid.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab12317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 21921037
WB 1/1000 - 1/10000. Predicted molecular weight: 155 kDa.
IHC-P Use at an assay dependent concentration.
IP Use a concentration of 1 - 4 mg/ml.

ターゲット情報

  • 機能
    Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.
  • 組織特異性
    Widely expressed. High expression in testis.
  • 配列類似性
    Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
    Contains 1 MBD (methyl-CpG-binding) domain.
    Contains 1 post-SET domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
    Contains 2 Tudor domains.
  • ドメイン
    The pre-SET, SET and post-SET domains are all required for methyltransferase activity. The 347-amino-acid insertion in the SET domain has no effect on the catalytic activity.
    Isoform 2 lacks all domains required for histone methyltransferase activity.
  • 細胞内局在
    Nucleus. Chromosome. Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AU022152 antibody
    • EC 2.1.1.43 antibody
    • ERG-associated protein with SET domain antibody
    • ESET antibody
    • H3 K9 HMTase 4 antibody
    • H3-K9-HMTase 4 antibody
    • H3-K9-HMTase4 antibody
    • Histone H3 K9 methyltransferase 4 antibody
    • Histone H3-K9 methyltransferase 4 antibody
    • Histone-lysine N-methyltransferase SETDB1 antibody
    • KG1T antibody
    • KIAA0067 antibody
    • KMT1E antibody
    • Lysine N-methyltransferase 1E antibody
    • MGC90670 antibody
    • mKIAA0067 antibody
    • SET domain bifurcated 1 antibody
    • Set domain protein, bifurcated, 1 antibody
    • SETB1_HUMAN antibody
    • Setdb1 antibody
    see all

画像



  • Predicted band size : 155 kDa
    Samples: Whole cell lysate (10 mcg for 3 and 4; 50 mcg for 1 and 2) from 293T cells that were mock transfected (1 and 2) or transfected with a SETDB1 expression construct (3 and 4).
  • ab12317 (4ug/ml) staining KMT1E in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of KMT1E in nuclear compartments of the germinal epithelium.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Whole cell lysate (2 mg) from 293T cells that were mock transfected (1 and 2) or transfected with a SETDB1 expression construct (3 and 4). Rabbit anti-SETDB1 antibodies; ab12317 (2 and 4) and a competitor's antiserum (1 and 3) were used at 1 mcg/mg lysate for IP. ab12317 was used at 0.2 mcg/ml for Western Blot. Detection: Chemiluminescence with exposure times of 1 to 5 minutes.

参考文献

This product has been referenced in:
  • Alarcon V  et al. The enzymes LSD1 and Set1A cooperate with the viral protein HBx to establish an active hepatitis B viral chromatin state. Sci Rep 6:25901 (2016). Read more (PubMed: 27174370) »
  • Hatanaka Y  et al. Histone chaperone CAF-1 mediates repressive histone modifications to protect preimplantation mouse embryos from endogenous retrotransposons. Proc Natl Acad Sci U S A 112:14641-6 (2015). ICC/IF ; Mouse . Read more (PubMed: 26546670) »

See all 17 Publications for this product

レビューと Q&A

Thank you for taking time to answer the additional questions. I am sorry to hear this antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and will provide us with vital information for monit...

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Thanks for your enquiry. There are two western blots on the datasheet for ab12317. I believe you are referring to the bottom image. I think there is a mistake in the annotation for this blot. 'Mock transfected' should refer to lanes 3 and 4 of this blo...

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Thank you for your enquiry. The predicted molecular weight of SETDB1 is 155kDa, based on the amino acid composition of the protein. It should be noted however that this protein is known to be phophorylated, as indicated on the Swissprot data page entry...

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The precise sequence used to generate ab12317 is proprietary. The epitope recognized by ab12317 is found between residues 1125 and 1175 of SwissProt entry Q15047 (http://us.expasy.org/cgi-bin/niceprot.pl?Q15047).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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