Anti-KMT1E / SETDB1 抗体 - ChIP Grade (ab12317)

製品の概要

  • 製品名Anti-KMT1E / SETDB1 antibody - ChIP Grade
    KMT1E / SETDB1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KMT1E / SETDB1 - ChIP Grade
  • アプリケーション適用あり: ChIP, ICC/IF, WB, IHC-P, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Immunogen was a synthetic peptide, which represented a portion of human SET domain, bifurcated 1 encoded within exon 19 (LocusLink ID 9869).

  • ポジティブ・コントロール
    • 293T cells.
  • 特記事項Centrifuge tube to remove product from lid.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab12317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 21921037
WB 1/1000 - 1/10000. Predicted molecular weight: 155 kDa.
IHC-P Use at an assay dependent concentration.
IP Use a concentration of 1 - 4 mg/ml.

ターゲット情報

  • 機能Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.
  • 組織特異性Widely expressed. High expression in testis.
  • 配列類似性Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
    Contains 1 MBD (methyl-CpG-binding) domain.
    Contains 1 post-SET domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
    Contains 2 Tudor domains.
  • ドメインThe pre-SET, SET and post-SET domains are all required for methyltransferase activity. The 347-amino-acid insertion in the SET domain has no effect on the catalytic activity.
    Isoform 2 lacks all domains required for histone methyltransferase activity.
  • 細胞内局在Nucleus. Chromosome. Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AU022152 antibody
    • EC 2.1.1.43 antibody
    • ERG-associated protein with SET domain antibody
    • ESET antibody
    • H3 K9 HMTase 4 antibody
    • H3-K9-HMTase 4 antibody
    • H3-K9-HMTase4 antibody
    • Histone H3 K9 methyltransferase 4 antibody
    • Histone H3-K9 methyltransferase 4 antibody
    • Histone-lysine N-methyltransferase SETDB1 antibody
    • KG1T antibody
    • KIAA0067 antibody
    • KMT1E antibody
    • Lysine N-methyltransferase 1E antibody
    • MGC90670 antibody
    • mKIAA0067 antibody
    • SET domain bifurcated 1 antibody
    • Set domain protein, bifurcated, 1 antibody
    • SETB1_HUMAN antibody
    • Setdb1 antibody
    see all

Anti-KMT1E / SETDB1 antibody - ChIP Grade 画像

  • Whole cell lysate (2 mg) from 293T cells that were mock transfected (1 and 2) or transfected with a SETDB1 expression construct (3 and 4). Rabbit anti-SETDB1 antibodies; ab12317 (2 and 4) and a competitor's antiserum (1 and 3) were used at 1 mcg/mg lysate for IP. ab12317 was used at 0.2 mcg/ml for Western Blot. Detection: Chemiluminescence with exposure times of 1 to 5 minutes.


  • Predicted band size : 155 kDa
  • ab12317 (4ug/ml) staining KMT1E in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of KMT1E in nuclear compartments of the germinal epithelium.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-KMT1E / SETDB1 antibody - ChIP Grade (ab12317) 使用論文

This product has been referenced in:
  • Calderon MR  et al. Ligand-dependent corepressor contributes to transcriptional repression by C2H2 zinc-finger transcription factor ZBRK1 through association with KRAB-associated protein-1. Nucleic Acids Res 42:7012-27 (2014). ChIP . Read more (PubMed: 24829459) »
  • Kao TH  et al. Ectopic DNMT3L triggers assembly of a repressive complex for retroviral silencing in somatic cells. J Virol 88:10680-95 (2014). Read more (PubMed: 24991018) »

See all 13 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (brain)
Permeabilization Yes - 0.5% Triton
Specification brain
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 01 2015

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
Sample Mouse Tissue sections (Ovary section)
Specification Ovary section
Permeabilization Yes - Trition
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jan 30 2015

Thank you for taking time to answer the additional questions. I am sorry to hear this antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and will provide us with vital information for monit...

Read More

Thanks for your enquiry. There are two western blots on the datasheet for ab12317. I believe you are referring to the bottom image. I think there is a mistake in the annotation for this blot. 'Mock transfected' should refer to lanes 3 and 4 of this blo...

Read More

Thank you for your enquiry. The predicted molecular weight of SETDB1 is 155kDa, based on the amino acid composition of the protein. It should be noted however that this protein is known to be phophorylated, as indicated on the Swissprot data page entry...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP on chip
Sample Mouse Cell lysate - nuclear (mouse ES cells)
Specification mouse ES cells
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Detection step Microarray
Positive control Competitor Antibody
Username

Dr. Steve Bilodeau

Verified customer

投稿 Oct 21 2009

The precise sequence used to generate ab12317 is proprietary. The epitope recognized by ab12317 is found between residues 1125 and 1175 of SwissProt entry Q15047 (http://us.expasy.org/cgi-bin/niceprot.pl?Q15047).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"