Anti-KMT1E / SETDB1 抗体 (ab12317)
Key features and details
- Rabbit polyclonal to KMT1E / SETDB1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-KMT1E / SETDB1 antibody
KMT1E / SETDB1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KMT1E / SETDB1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide within Human KMT1E/ SETDB1 aa 1100-1200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q15047 -
ポジティブ・コントロール
- WB: HeLa and HEK293T whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified using the peptide immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12317の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 155 kDa.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 155 kDa. |
ターゲット情報
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機能
Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. -
組織特異性
Widely expressed. High expression in testis. -
配列類似性
Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 1 MBD (methyl-CpG-binding) domain.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain.
Contains 2 Tudor domains. -
ドメイン
The pre-SET, SET and post-SET domains are all required for methyltransferase activity. The 347-amino-acid insertion in the SET domain has no effect on the catalytic activity.
Isoform 2 lacks all domains required for histone methyltransferase activity. -
細胞内局在
Nucleus. Chromosome. Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin. - Information by UniProt
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参照データベース
- Entrez Gene: 9869 Human
- Entrez Gene: 84505 Mouse
- Omim: 604396 Human
- SwissProt: Q15047 Human
- SwissProt: O88974 Mouse
- Unigene: 643565 Human
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別名
- AU022152 antibody
- EC 2.1.1.43 antibody
- ERG-associated protein with SET domain antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (27)
ab12317 は 27 報の論文で使用されています。
- Guo E et al. WEE1 inhibition induces anti-tumor immunity by activating ERV and the dsRNA pathway. J Exp Med 219:N/A (2022). PubMed: 34825915
- Barral A et al. SETDB1/NSD-dependent H3K9me3/H3K36me3 dual heterochromatin maintains gene expression profiles by bookmarking poised enhancers. Mol Cell 82:816-832.e12 (2022). PubMed: 35081363
- Saavedra F et al. JMJD1B, a novel player in histone H3 and H4 processing to ensure genome stability. Epigenetics Chromatin 13:6 (2020). PubMed: 32070414
- Nava MM et al. Heterochromatin-Driven Nuclear Softening Protects the Genome against Mechanical Stress-Induced Damage. Cell 181:800-817.e22 (2020). PubMed: 32302590
- Krasnopolsky S et al. Genome-wide CRISPR knockout screen identifies ZNF304 as a silencer of HIV transcription that promotes viral latency. PLoS Pathog 16:e1008834 (2020). PubMed: 32956422