製品の概要

  • 製品名
    Anti-KMT1B / SUV39H2 antibody
    KMT1B / SUV39H2 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to KMT1B / SUV39H2
  • アプリケーション
    適用あり: ChIP, WBmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to Human KMT1B/ SUV39H2 aa 398-410 (C terminal).
    Sequence:

    CKCGAVTCRGYLN


    Database link: Q9H5I1
    (Peptide available as ab23082)

  • ポジティブ・コントロール
    • Rat Kidney Lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab5264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP Use at an assay dependent concentration. PubMed: 21060834
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 47 kDa.Can be blocked with Human KMT1B / SUV39H2 peptide (ab23082).

ターゲット情報

  • 機能
    Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher order chromatin organization during spermatogenesis.
  • 配列類似性
    Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
    Contains 1 chromo domain.
    Contains 1 post-SET domain.
    Contains 1 pre-SET domain.
    Contains 1 SET domain.
  • ドメイン
    Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin.
  • 細胞内局在
    Nucleus. Chromosome > centromere. Associates with centromeric constitutive heterochromatin.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ23414 antibody
    • H3 K9 HMTase 2 antibody
    • H3-K9-HMTase 2 antibody
    • Histone H3 K9 methyltransferase 2 antibody
    • Histone H3-K9 methyltransferase 2 antibody
    • Histone lysine N methyltransferase H3 lysine 9 specific 2 antibody
    • Histone lysine N methyltransferase SUV39H2 antibody
    • Histone-lysine N-methyltransferase SUV39H2 antibody
    • KMT1B antibody
    • Lysine N methyltransferase 1B antibody
    • Lysine N-methyltransferase 1B antibody
    • sSuppressor of variegation 3 9 homolog 2 (Drosophila) antibody
    • Su(var)3 9 Drosophila homolog of 2 antibody
    • Su(var)3 9 homolog 2 antibody
    • Su(var)3-9 homolog 2 antibody
    • Suppressor of variegation 3 9 homolog 2 antibody
    • Suppressor of variegation 3-9 homolog 2 antibody
    • Suv39h2 antibody
    • SUV92_HUMAN antibody
    see all

画像

  • Anti-KMT1B / SUV39H2 antibody (ab5264) at 1 µg/ml + Rat Kidney Lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 47 kDa

    Primary incubation 1 hour

参考文献

This product has been referenced in:
  • Yang G  et al. The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction. Nat Commun 8:14941 (2017). ChIP . Read more (PubMed: 28361889) »
  • Salton M  et al. Identification by high-throughput imaging of the histone methyltransferase EHMT2 as an epigenetic regulator of VEGFA alternative splicing. Nucleic Acids Res 42:13662-73 (2014). ChIP ; Human . Read more (PubMed: 25414343) »

See all 3 Publications for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - nuclear (MEF)
Loading amount
100 µg
Specification
MEF
Gel Running Conditions
Non-reduced Denaturing (gel 10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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Verified customer

投稿 Mar 12 2009

Application
ChIP on chip
Sample
Mouse Cell lysate - nuclear (MEF)
Specification
MEF
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldhehyde
Detection step
Microarray
Username

Abcam user community

Verified customer

投稿 May 30 2008

Thank you for your enquiry. To our knowledge, ab3659 has yet to be tested in IF. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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