Anti-KMT1A / SUV39H1 抗体 [42AT239.96.72] (ab3659)


  • 製品名Anti-KMT1A / SUV39H1 antibody [42AT239.96.72]
    KMT1A / SUV39H1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [42AT239.96.72] to KMT1A / SUV39H1
  • アプリケーション適用あり: ELISA, WBmore details
    適用なし: ICC/IF
  • 種交差性
    交差種: Human
  • 免疫原

    This antibody was raised using purified recombinant fusion protein encoding N-terminal of human SUV39H1.



Our Abpromise guarantee covers the use of ab3659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 47 kDa (predicted molecular weight: 47.9 kDa).
  • 追加情報Is unsuitable for ICC/IF.
  • ターゲット情報

    • 機能Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. Also weakly methylates histone H1 (in vitro). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiation, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length. Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. Recruited by the large PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1, contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation.
    • 配列類似性Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
      Contains 1 chromo domain.
      Contains 1 post-SET domain.
      Contains 1 pre-SET domain.
      Contains 1 SET domain.
    • 発生段階Accumulates during mitosis at centromeres during prometaphase, but dissociates from the centromere at the meta- to anaphase transition.
    • ドメインAlthough the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin.
      In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster.
    • 翻訳後修飾Phosphorylated on serine residues, and to a lesser degree, on threonine residues. The phosphorylated form is stabilized by SBF1 and is less active in its transcriptional repressor function.
      Acetylated at Lys-266, leading to inhibition of enzyme activity. SIRT1-mediated deacetylation relieves this inhibition.
    • 細胞内局在Nucleus. Nucleus lamina. Nucleus, nucleoplasm. Chromosome, centromere. Associates with centromeric constitutive heterochromatin.
    • Information by UniProt
    • 参照データベース
    • 別名
      • H3 K9 HMTase1 antibody
      • H3-K9-HMTase 1 antibody
      • Histone H3-K9 methyltransferase 1 antibody
      • Histone H3-K9 methyltransferase1 antibody
      • Histone lysine N methyltransferase, H3 lysine 9 specific 1 antibody
      • Histone-lysine N-methyltransferase SUV39H1 antibody
      • KMT1 A antibody
      • KMT1A antibody
      • Lysine N methyltransferase 1A antibody
      • Lysine N-methyltransferase 1A antibody
      • MG44 antibody
      • mIS6 antibody
      • Position-effect variegation 3-9 homolog antibody
      • Su(var)3 9 homolog 1 antibody
      • Su(var)3-9 homolog 1 antibody
      • Suppressor of variegation 3 9 homolog 1 (Drosophila) antibody
      • Suppressor of variegation 3-9 homolog 1 antibody
      • SUV39 H1 antibody
      • SUV39H antibody
      • SUV39H1 antibody
      • SUV91_HUMAN antibody
      see all

    Anti-KMT1A / SUV39H1 antibody [42AT239.96.72] 画像

    • Predicted band size : 47.9 kDa

      Western analysis of extracts from 293 cells using SUV39H1 antibody (ab3659).

    Anti-KMT1A / SUV39H1 antibody [42AT239.96.72] (ab3659) 使用論文

    ab3659 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application ChIP
    Sample Human Cell lysate - whole cell (HeLa)
    Specification HeLa
    Type Cross-linking (X-ChIP)
    Duration of cross-linking step: 0 second(s)
    Specification of the cross-linking agent: Formaldehyde
    Detection step Other

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    投稿 Jul 07 2006

    Your protocol looks fine and I have only one suggestion which may allow you to detect a band. We recommend that the antibody is used at a dilution of between 1:100 - 1:500. You have used it at the far end of this range. I would suggest running another ...

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    From your previous correspondence with my colleagues, I see that you enquired at the end of April whether you could have your money back if this antibody did not work as we specify on the datasheet. We shipped the product to you at the beginning of May...

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    Thank you for your enquiry. There is approximately 1-2 ug per lane of protein for the SuV39h1 antibody staining experiment. Abcam policy dictates that if a product does not performed as stated on the datasheet, we will offer a full reimbursement or pro...

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    Thank you for your enquiry. The supplier of this antibody has informed me that ab3659 has not been tested on mouse. I apologize, as it says on the online datasheet that this antibody cross-reacts with both human and mouse and I will correct this. If yo...

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