製品の概要

  • 製品名Anti-Klotho antibody
    Klotho 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Klotho
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide of 17 amino acids (Mouse) (C terminal).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab18131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 116 kDa.
ELISA 1/10000 - 1/100000.

ターゲット情報

  • 機能May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1.
    The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.
  • 組織特異性Present in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney.
  • 関連疾患Defects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC) [MIM:211900]. A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement.
  • 配列類似性Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.
  • ドメインContains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 239, and the second one lacks the essential Glu active site residue at position 872.
  • 翻訳後修飾N-glycosylated.
  • 細胞内局在Secreted and Cell membrane. Isoform 1 shedding leads to a soluble peptide.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Kl antibody
    • KLOT_HUMAN antibody
    • Klotho peptide antibody

Anti-Klotho antibody (ab18131) 使用論文

This product has been referenced in:
  • Labrie V  et al. Serine racemase is associated with schizophrenia susceptibility in humans and in a mouse model. Hum Mol Genet 18:3227-43 (2009). WB ; Mouse . Read more (PubMed: 19483194) »
  • Li Q  et al. Deficiency of insulin-like growth factor 1 reduces sensitivity to aging-associated cardiomyocyte dysfunction. Rejuvenation Res 11:725-33 (2008). WB ; Mouse . Read more (PubMed: 18729805) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (Hippocampus)
Loading amount 30 µg
Specification Hippocampus
Gel Running Conditions Non-reduced Denaturing (14% gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Gareth Bowen

Verified customer

投稿 Apr 25 2013

Thank you very much for your interest in ab18131.
To our knowledge, ab18131 has not been tested in Human. Therefore, I can offer a discount off a future purchase if you buy ab18131 now, test it in Human and submit feedback to us in the form of an ...

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Thank you for your inquiry and I apologize for the delay in this response. The C-terminal immunizing peptide used to generate ab18131 is based on the 1014 amino acid sequence of membrane-bound Klotho. This antibody should only recognize the membr...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (choroid plexus, kidney)
Specification choroid plexus, kidney
Fixative Paraformaldehyde
Antigen retrieval step None
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Jan 27 2006

Thank you for your enquiry. The antibody orignates outside of Abcam and the originator of the antibody never reveals an immunogen peptide sequence. All I can provide is: "17aa peptide sequence near the C-terminus of mouse Klotho." I hope this inf...

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Thank you for your email. We do not carry klotho recombinant protein. I did a Google search, but unfortunately couldn't see any companies that do sell it. I'm still looking into your original question regarding ab18131 and will get back to you once...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"