Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
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Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1.25 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Anti-KLHL26 antibody 画像
Western blot - KLHL26 antibody (ab48972)
Lane 1 :
MARKERLane 2 :
Anti-KLHL26 antibody (ab48972) at 1.25 µg/mlLane 1 :
As aboveLane 2 :
Jurkat cell lysate at 10 µg with 5% skimmed milk in PBS.SecondaryLane 2 :
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size :
68 kDaObserved band size :
70 kDa (why is the actual band size different from the predicted?
Anti-KLHL26 antibody (ab48972) 使用論文
has not yet been referenced specifically in any publications.
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