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A region within synthetic peptide: QSEDFNSLSK DTLLDLISSD ELETEDERVV FEAILQWVKH DLEPRKVHLP, corresponding to N terminal amino acids 181-230 of Human KLHL25
Our Abpromise guarantee covers the use of ab55953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3125 µg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
ELISA titre using peptide based assay:1:1562500.
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling KLHL25 with ab55953 at 4-8µg/ml Arrows indicate postively labelled neural cells. Magnification: 400X.
ab55953 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"