Anti-KLHL21 抗体 (ab111604)
Key features and details
- Rabbit polyclonal to KLHL21
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-KLHL21 antibody -
製品の詳細
Rabbit polyclonal to KLHL21 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow -
免疫原
Recombinant fragment corresponding to a region within amino acids 345-520 of Human KLHL21
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ポジティブ・コントロール
- WB: A549, HeLa, Jurkat and Raji whole cell lysates IHC-P: Hepatoma tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab111604の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 67 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with 10mM citrate buffer pH 6.0 or Tris-EDTA buffer pH 8.0 before commening with IHC staining protocol.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 67 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with 10mM citrate buffer pH 6.0 or Tris-EDTA buffer pH 8.0 before commening with IHC staining protocol. |
ターゲット情報
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機能
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for efficient chromosome alignment and cytokinesis. The BCR(KLHL21) E3 ubiquitin ligase complex regulates localization of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain.
Contains 6 Kelch repeats. -
細胞内局在
Cytoplasm > cytoskeleton > spindle. Localizes to the spindle midzone and targets CUL3 to this region. - Information by UniProt
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参照データベース
- Entrez Gene: 506632 Cow
- Entrez Gene: 9903 Human
- Entrez Gene: 242785 Mouse
- Entrez Gene: 313743 Rat
- SwissProt: Q08DS0 Cow
- SwissProt: Q9UJP4 Human
- SwissProt: Q3U410 Mouse
- SwissProt: D4A2K4 Rat
see all -
別名
- Kelch-like protein 21 antibody
- KIAA0469 antibody
- KLH21_HUMAN antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab111604 は 2 報の論文で使用されています。
- Wang J et al. EZH2 Regulates the Correlation between Skin Regeneration and the Duration of Mechanical Stretch. J Invest Dermatol 141:894-902.e9 (2021). PubMed: 33069730
- Mei ZZ et al. Kelch-like Protein 21 (KLHL21) Targets I?B Kinase-ß to Regulate Nuclear Factor ?-Light Chain Enhancer of Activated B Cells (NF-?B) Signaling Negatively. J Biol Chem 291:18176-89 (2016). PubMed: 27387502