製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to KLHL20
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length KLHL20 protein (Human)

  • ポジティブ・コントロール
    • KLHL20 transfected 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab67682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 68 kDa).

ターゲット情報

  • 機能
    Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in interferon response. The BCR(KLHL20) E3 ubiquitin ligase complex mediates the ubiquitination of DAPK1, leading to its degradation by the proteasome, thereby acting as a negative regulator of apoptosis. Also acts as a regulator of endothelial migration during angiogenesis by controlling the activation of Rho GTPases.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Contains 1 BACK (BTB/Kelch associated) domain.
    Contains 1 BTB (POZ) domain.
    Contains 6 Kelch repeats.
  • 細胞内局在
    Cytoplasm > perinuclear region. Nucleus. Localizes in the perinuclear region in normal conditions. Following IFN-alpha or IFN-gamma treatment, it is relocalized and sequestrated to the PML nuclear bodies, preventing DAPK1 ubiquitination.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Kelch like ECT2 interacting protein antibody
    • Kelch like protein 20 antibody
    • Kelch like protein X antibody
    • Kelch motif containing protein antibody
    • Kelch-like ECT2-interacting protein antibody
    • Kelch-like protein 20 antibody
    • Kelch-like protein X antibody
    • KHLHX antibody
    • KLEIP antibody
    • KLH20_HUMAN antibody
    • KLHL20 antibody
    • KLHLX antibody
    • RP3-383J4.3 antibody
    see all

Anti-KLHL20 antibody 画像

  • All lanes : Anti-KLHL20 antibody (ab67682) at 1/500 dilution

    Lane 1 : KLHL20 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 68 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

プロトコール

Anti-KLHL20 antibody (ab67682) 使用論文

ab67682 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Human osteosarcoma U2OS cells)
Loading amount
90 µg
Specification
Human osteosarcoma U2OS cells
Gel Running Conditions
Reduced Denaturing (4-18% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Apr 11 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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