Anti-KLHL20 抗体 (ab67682)
Key features and details
- Mouse polyclonal to KLHL20
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-KLHL20 antibody -
製品の詳細
Mouse polyclonal to KLHL20 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human KLHL20. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- KLHL20 transfected 293T cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab67682の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 68 kDa).
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 68 kDa). |
ターゲット情報
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機能
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in interferon response. The BCR(KLHL20) E3 ubiquitin ligase complex mediates the ubiquitination of DAPK1, leading to its degradation by the proteasome, thereby acting as a negative regulator of apoptosis. Also acts as a regulator of endothelial migration during angiogenesis by controlling the activation of Rho GTPases. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain.
Contains 6 Kelch repeats. -
細胞内局在
Cytoplasm > perinuclear region. Nucleus. Localizes in the perinuclear region in normal conditions. Following IFN-alpha or IFN-gamma treatment, it is relocalized and sequestrated to the PML nuclear bodies, preventing DAPK1 ubiquitination. - Information by UniProt
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参照データベース
- Entrez Gene: 27252 Human
- SwissProt: Q9Y2M5 Human
- Unigene: 495035 Human
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別名
- Kelch like ECT2 interacting protein antibody
- Kelch like protein 20 antibody
- Kelch like protein X antibody
see all
画像
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All lanes : Anti-KLHL20 antibody (ab67682) at 1/500 dilution
Lane 1 : KLHL20 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 68 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (0)
ab67682 は論文での使用が確認できていません。