製品の概要

  • 製品名
    Anti-KLHL17 antibody
  • 製品の詳細
    Rabbit polyclonal to KLHL17
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence: AGAWESVAPM NIRRSTHDLV AMDGWLYAVG GNDGSSSLNS IEKYNPRTNK WVAASCMFTR RSSVGVAVLE LLNFPPPSSP TLSVS, corresponding to amino acids 554-638 of Human KLHL17.

  • ポジティブ・コントロール
    • Human testis tissue; RT 4, U 51 MG and Human plasma lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab122164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

ターゲット情報

  • 機能
    Substrate-recognition component of some cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex. The BCR(KLHL17) mediates the ubiquitination and subsequenct degradation of GLUR6. May play a role in the actin-based neuronal function.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Contains 1 BACK (BTB/Kelch associated) domain.
    Contains 1 BTB (POZ) domain.
    Contains 6 Kelch repeats.
  • 細胞内局在
    Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Postsynaptic density.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Actinfilin antibody
    • AL022703 antibody
    • BC058738 antibody
    • kelch like 17 antibody
    • kelch like family member 17 antibody
    • kelch like protein 17 antibody
    • Kelch-like protein 17 antibody
    • KLH17_HUMAN antibody
    • Klhl17 antibody
    • RP11-54O7.6 antibody
    see all

画像

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122164 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122164 at 1/100 dilution staining KLHL17 in Paraffin-embedded Human testis tissue by Immunohistochemistry.
  • All lanes : Anti-KLHL17 antibody (ab122164) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

参考文献

ab122164 has not yet been referenced specifically in any publications.

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