Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
Widely expressed, with high levels in skeletal muscle, pancreas and liver. Undetectable in peripheral blood leukocytes.
Contains 6 Kelch repeats.
Information by UniProt
- Kelch domain containing 1 antibody
- Kelch domain containing protein 1 antibody
- Kelch domain-containing protein 1 antibody
Anti-KLHDC1 antibody 画像
Western blot - KLHDC1 antibody (ab75301)
Anti-KLHDC1 antibody (ab75301) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size :
47 kDaObserved band size :
39 kDa (why is the actual band size different from the predicted?
)Additional bands at :
30 kDa. We are unsure as to the identity of these extra bands.
Anti-KLHDC1 antibody (ab75301) 使用論文
has not yet been referenced specifically in any publications.
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