製品の概要

  • 製品名
    Anti-KLF4 antibody [56CT5.1.6]
    KLF4 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [56CT5.1.6] to KLF4
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Recombinant fragment
  • 免疫原

    Recombinant fragment corresponding to Human KLF4 aa 10-479.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/10 - 1/50.
WB 1/2000. Predicted molecular weight: 50 kDa.

ターゲット情報

  • 機能
    Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
  • 配列類似性
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • ドメイン
    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 別名
    • Endothelial Kruppel like zinc finger protein antibody
    • Epithelial zinc finger protein EZF antibody
    • EZF antibody
    • GKLF antibody
    • gut Kruppel-like factor antibody
    • Gut-enriched krueppel-like factor antibody
    • KLF antibody
    • KLF4 antibody
    • KLF4_HUMAN antibody
    • Krueppel-like factor 4 antibody
    • Kruppel like factor 4 (Epithelial zinc finger protein EZF) (Gut enriched Krueppel like factor) antibody
    • Kruppel like factor 4 (gut) antibody
    see all

Anti-KLF4 antibody [56CT5.1.6] 画像

  • Anti-KLF4 antibody [56CT5.1.6] (ab75486) at 1/200 dilution + KLF4 recombinant protein

    Predicted band size : 50 kDa
  • Immunoflurescence analysis of ab75486 (5 μg/ml). HeLa cells transfected with pMX constructs of human KLF4 (top) and NIH3T3 cells transfected with pMX constructs of mouse KLF4 (bottom) were analyzed at approximately 62 hours after transfection.

Anti-KLF4 antibody [56CT5.1.6] (ab75486) 使用論文

This product has been referenced in:
  • Lee E  et al. DNMT1 Regulates Epithelial-Mesenchymal Transition and Cancer Stem Cells, Which Promotes Prostate Cancer Metastasis. Neoplasia 18:553-66 (2016). Human . Read more (PubMed: 27659015) »
  • Park SJ  et al. MacroH2A1 downregulation enhances the stem-like properties of bladder cancer cells by transactivation of Lin28B. Oncogene 35:1292-301 (2016). WB ; Human . Read more (PubMed: 26028027) »

See all 9 Publications for this product

Product Wall

Application
Western blot
Sample
Chinese Hamster Cell lysate - whole cell (CHO-K1)
Loading amount
35 µg
Specification
CHO-K1
Treatment
transfected with mouse KLF4 (no tag)
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Nov 09 2012

According to our records, ab75486 was proving difficult to use inWB and we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand ...

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Thank you for contacting Abcam regarding ab75486.


I am sorry that you have been experiencing difficulties with this antibody in WB. I am hoping you can provide some additional information to help me resolve the issues you are having wit...

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Thank you for enquiry and your interest in our products.

Currently, testing our antibodies in EMSA is not part of the standard QC process. Most of our products are characterized regularly in WB, IHC, ICC, ELISA, Flow cytometry, ChIP.
Read More

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (cell type)
Specification
cell type
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Yunhui Cheng

Verified customer

投稿 Mar 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Jennifer Schnabel

Verified customer

投稿 Sep 23 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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