製品の概要

  • 製品名Anti-KLF15 antibody
    KLF15 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to KLF15
  • アプリケーション適用あり: IP, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Pig
  • 免疫原

    Synthetic peptide: VDHLLPVDENFSS, corresponding to N terminal amino acids 2-14 of Human KLF15.

  • ポジティブ・コントロール
    • Human Liver or HepG2 lysate
  • 特記事項

    Principal Names – KLF15; Kruppel-like factor 15; KKLF; KKLF protein; kidney-enriched Kruppel-like factor Official Gene Symbol - KLF15 GenBank Accession Number – NP_054798 LocusLink ID - 28999 (human); 66277 (mouse); 85497 (rat)

     

    We no longer guarantee this product for mouse because of recent complaints. The immunogen however shares 92% sequence homology with mouse so we still predict the antibody will cross-react. We will welcome any feedback from customers.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab2647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration. PubMed: 24407292
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).Can be blocked with Human KLF15 peptide (ab22927).

Approx 48kDa band observed in lysates of hepatoblastoma cell line HepG2 (calculated MW of 44.0kDa according to NP_054798.1). Older batches detected a band of approximately 65 kDa in Human Liver lysate(predicted molecular weight: 46 kDa). Note the higher than expected size is in agreement with previous Western results (Mol Cell Biol 2000 Oct;20(19):7319-31).

ターゲット情報

Anti-KLF15 antibody 画像



  • Predicted band size : 44 kDa


    ab2647 staining (1 µg/ml) of Mouse skeletal muscle lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.

Anti-KLF15 antibody (ab2647) 使用論文

This product has been referenced in:
  • Prosdocimo DA  et al. Kruppel-like factor 15 is a critical regulator of cardiac lipid metabolism. J Biol Chem 289:5914-24 (2014). WB, IP ; Mouse, Human . Read more (PubMed: 24407292) »
  • Mallipattu SK  et al. Kruppel-like Factor 15 (KLF15) Is a Key Regulator of Podocyte Differentiation. J Biol Chem 287:19122-35 (2012). WB ; Mouse . Read more (PubMed: 22493483) »

See all 4 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab2647.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"