製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to KLF13
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human KLF13.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following nuclear lysates: HeLa; Jurkat; HepG2; MCF7.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab88536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 31 kDa).

ターゲット情報

  • 機能
    Represses transcription by binding to the BTE site, a GC-rich DNA element, in competition with the activator SP1. It also represses transcription by interacting with the corepressor Sin3A and HDAC1. Activates RANTES expression in T-cells.
  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Belongs to the Sp1 C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • ドメイン
    The Ala/Pro-rich domain may contain discrete activation and repression subdomains and also can mediate protein-protein interactions.
  • 翻訳後修飾
    Phosphorylated.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Basic transcription element binding protein 3 antibody
    • Basic transcription element-binding protein 3 antibody
    • BTE binding protein 3 antibody
    • BTE-binding protein 3 antibody
    • BTEB3 antibody
    • C2 H2 zinc finger protein antibody
    • FKLF 2 antibody
    • FKLF2 antibody
    • Klf13 antibody
    • KLF13_HUMAN antibody
    • Krueppel-like factor 13 antibody
    • Kruppel like factor 13 antibody
    • Novel Sp1 like zinc finger transcription factor antibody
    • Novel Sp1-like zinc finger transcription factor 1 antibody
    • NSLP1 antibody
    • RANTES factor of late activated T lymphocytes 1 antibody
    • RANTES factor of late activated T-lymphocytes 1 antibody
    • RFLAT 1 antibody
    • RFLAT-1 antibody
    • RFLAT1 antibody
    • Transcription factor BTEB3 antibody
    • Transcription factor NSLP1 antibody
    see all

画像

  • All lanes : Anti-KLF13 antibody (ab88536) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 2 : Jurkat nuclear extract lysate (ab14844)
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate
    Lane 4 : MCF7 nuclear extract lysate (ab14860)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa,45 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

プロトコール

参考文献

ab88536 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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