製品の概要

  • 製品名Anti-KLC3 antibody
    KLC3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to KLC3
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Macaque Monkey, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human KLC3.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Human spleen; Human small intestine; Human ovary; Human testis.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab101188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport.
  • 配列類似性Belongs to the kinesin light chain family.
    Contains 5 TPR repeats.
  • ドメインThe heptad repeat (HR) motif is sufficient for interaction with kinesin heavy (KHL) chains and ODF1. The TPR region is involved in mitochondrial binding.
  • 細胞内局在Cytoplasm > cytoskeleton. Elongating spermatid tail midpiece, localized in outer dense fibers (ODFs) and associates with mitochondria.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Kinesin light chain 2 antibody
    • Kinesin light chain 3 antibody
    • KLC2 antibody
    • KLC2 like antibody
    • KLC2-like antibody
    • KLC2L antibody
    • KLC3 antibody
    • KLC3_HUMAN antibody
    • KLCt antibody
    • KNS2B antibody
    see all

Anti-KLC3 antibody 画像

  • All lanes : Anti-KLC3 antibody (ab101188) at 1 µg/ml

    Lane 1 : Human spleen tissue lysate - total protein (ab29699)
    Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 3 : Human ovary tissue lysate - total protein (ab30222)
    Lane 4 : Human testis tissue lysate - total protein (ab30257)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 29 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 14 minutes
  • ICC/IF image of ab101188 stained U87MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab101188, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-KLC3 antibody (ab101188) 使用論文

ab101188 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab101188.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"