All tags Multiplex miRNA assays MicroRNA profiling direct from biofluids, using Firefly™ particle technology

MicroRNA profiling direct from biofluids, using Firefly™ particle technology

MicroRNA profiling direct from plasma or serum, without RNA purification. Reduce variability, simplify workflow and conserve your sample.


​The Multiplex Circulating miRNA Assay allows miRNA profiling direct from crude biofluids, including plasma, serum and exosomes, without the need for RNA purification. Find out how miRNA profiling direct from biofluids can benefit your experiment:

Reduced variability. Remove the unreliable RNA purification step in your sample preparation and get more consistent data.

Simplified workflow. Take less time from sample to data. RNA extraction adds significantly to the steps required to profile miRNAs across multiple samples. The Multiplex Circulating miRNA Assay with crude biofluids simplifies the workflow and cuts down assay time.

Smaller sample input. Profile miRNAs direct from as little as 10–40 μl crude biofluids. With the low abundance of miRNAs in biofluids, miRNA analysis typically requires a large quantity of biofluid for RNA extraction. Cut out this step to save your sample.

See the data for yourself

In this technical note, we demonstrate how this assay can be used for miRNA profiling with PCR sensitivity directly from as little as 20 µL of serum or plasma with no need for RNA purification, or from less than 100 pg of purified RNA. With our easy to use bioinformatics tools, we demonstrate that consistent miRNA profiles are obtained from plasma obtained using a variety of blood collection methods, including heparin.

Read the technical note in full

miRNA profiling in crude digests versus extracted DNA. There was a close correlation between miRNA expression profiles obtained from purified miRNA and those obtained from crude serum.


References

  • Chapin SC, Pregibon DC and Doyle PS (2011). Rapid mircoRNA profiling on encoded gel microparticles. Angew Chem Int Ed, 50, 2289-93.
  • Chapin SC, Pregibon DC and Doyle PS (2009). High-throughput flow alignment of barcoded hydrogel microparticles. Lab Chip, 9, 3100-9.
  • Dendukuri D, Pregibon DC, Collins J, Hatton TA and Doyle PS (2006). Continuous flow lithography for high-throughput microparticle synthesis. Nat Mater, 5, 365-9.
  • Pregibon DC and Doyle PS (2009). Optimization of encoded hydrogel particles for nucleic acid quantification. Anal Chem, 81, 4873-81.
  • Prefibon DC, Toner M and Doyle PS (2007). Multifunctional encoded particles for high-throughput biomolecule analysis. Science​, 315, 1393-96.