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ELISA kits to mouse targets may be less reproducible, specific and sensitive when compared to other species due to the use of batch-specific polyclonal antibodies. However, the use of a mouse monoclonal antibody to mouse targets may nevertheless generate an unacceptable level of background resulting in a less sensitive assay.
With the rabbit as a host, the monoclonal antibody produced to mouse targets is less immunogenic in mouse or rat. You can then use rabbit monoclonal antibodies in mouse models without worrying about nonspecific signal due to native mouse Ig cross-reactivity.
Table 1: Mouse IL-1a SimpleStep® ELISA kit (ab199076) made with a pair of rabbit monoclonal antibodies (ab210893) shows superior performance in sensitivity and assay precision over a standard ELISA kit made with non-rabbit monoclonal antibodies.
|Product||Range (pg/mL)||Sensitivity (pg/mL)||Intra-assay precision||Inter-assay precision|
Mouse IL-1a SimpleStep ELISA kit (ab199076)
|4.69 - 300||0.62||2.31%||4.78%|
|Mouse IL-1a ELISA kit (ab113344)||12.23 - 3000||10||10%||10%|