All tags elisa-matched-antibody-pairs-and-multiplex-immunoassays Elevate the quality of your mouse ELISA data

Elevate the quality of your mouse ELISA data

Rabbit monoclonal antibody pair provides low background with mouse samples

ELISA kits to mouse targets may be less reproducible, specific and sensitive when compared to other species due to the use of batch-specific polyclonal antibodies. However, the use of a mouse monoclonal antibody to mouse targets may nevertheless generate an unacceptable level of background resulting in a less sensitive assay.

With the rabbit as a host, the monoclonal antibody produced to mouse targets is less immunogenic in mouse or rat. You can then use rabbit monoclonal antibodies in mouse models without worrying about nonspecific signal due to native mouse Ig cross-reactivity.

Table 1: Mouse IL-1a SimpleStep® ELISA kit (ab199076) made with a pair of rabbit monoclonal antibodies (ab210893) shows superior performance in sensitivity and assay precision over a standard ELISA kit made with non-rabbit monoclonal antibodies.

ProductRange (pg/mL)Sensitivity (pg/mL)Intra-assay precisionInter-assay precision

Mouse IL-1a SimpleStep ELISA kit (ab199076)

4.69 - 3000.622.31%4.78%
Mouse IL-1a ELISA kit (ab113344)12.23 - 30001010%10%

Try our mouse IL-1a SimpleStep ELISA kit (ab199076) or try to set your own ELISA up with mouse IL-1a matched antibody pair kit (ab210893).