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miRNA | Choosing an assay

Choosing a multiplex miRNA assay

Select the correct multiplex assay for your miRNA profiling experiment.

Using Firefly™ particle technology, we have developed the Multiplex Cellular miRNA Assay for purified RNA, and the Multiplex Circulating miRNA Assay available for crude biofluids and purified RNA.

The assay most appropriate for your research depends on the nature of the samples you wish to test.

Already know what you want? Order your assay now.

Multiplex Circulating miRNA Assay: miRNA profiling direct from crude biofluid

Use the Multiplex Circulating miRNA Assay to:

  • Detect RNAs direct from crude biofluids
  • Work with limited sample availability 
  • Profile low abundance miRNAs

​ The Multiplex Circulating miRNA Assay can be used to detect miRNA with high specificity and reproducibility from a sample input of just 100 pg purified RNA or 20 μl biofluid. Whereas miRNA profiling protocols often include an RNA purification step, which can lead to biases in target recovery, this assay is ideal for the detection of miRNAs direct from crude biofluids including plasma and serum, regardless of purity.

Please note: The Multiplex Circulating miRNA Assay is available as two separate kits, one each for purified RNA and crude biofluids.

​​Download our assay workflow for Multiplex miRNA Circulating Crude Biofluid Assay

​​Download our assay workflow for Multiplex miRNA Circulating Purified RNA Assay


Multiplex Cellular miRNA Assay: for ease of workflow using purified RNA

Use the Multiplex Cellular miRNA Assay to:

  • Detect miRNAs in samples not limited in availability
  • Profile miRNAs with increased workflow simplicity

Unlike other approaches including qPCR and RNA-seq, the Multiplex Cellular miRNA Assay does not involve reverse transcription, library preparation or amplification, dramatically simplifying the workflow. The assay takes 3.5–4.5 hours from sample to data, depending on how many samples are processed in parallel.

This assay requires input of >500 ng of purified RNA.

Download our assay workflow for Multiplex Cellular miRNA Assay


Choosing a miRNA assay