製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to KIR2DL4
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human KIR2DL4.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human spleen tissue lysate as well as the following whole cell lysates: MDA MB 231; MDA MB 361; HeLa.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab100996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 41 kDa).

ターゲット情報

  • 機能
    Receptor on natural killer (NK) cells for HLA-C alleles. Inhibits the activity of NK cells thus preventing cell lysis.
  • 配列類似性
    Belongs to the immunoglobulin superfamily.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD_antigen=CD158d antibody
    • CD158 antigen like family member D antibody
    • CD158 antigen-like family member D antibody
    • CD158d antibody
    • G9P antibody
    • KI2L4_HUMAN antibody
    • Killer cell immunoglobulin like receptor 2DL4 antibody
    • Killer cell immunoglobulin like receptor, two domains, long cytoplasmic tail, 4 antibody
    • Killer cell immunoglobulin-like receptor 2DL4 antibody
    • Killer cell inhibitory receptor 103AS antibody
    • Killer Ig receptor antibody
    • KIR 103AS antibody
    • KIR-103AS antibody
    • KIR103 antibody
    • KIR103AS antibody
    • KIR2DL4 antibody
    • MHC class I NK cell receptor KIR103AS antibody
    • Natural killer cell inhibitory receptor antibody
    • NK cell receptor antibody
    • NK cell receptor; natural killer cell inhibitory receptor antibody
    • OTTHUMP00000068612 antibody
    • OTTHUMP00000068614 antibody
    • OTTHUMP00000068615 antibody
    see all

画像

  • All lanes : Anti-KIR2DL4 antibody (ab100996) at 1 µg/ml

    Lane 1 : Human spleen tissue lysate - total protein (ab29699)
    Lane 2 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

プロトコール

参考文献

ab100996 has not yet been referenced specifically in any publications.

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