製品の概要

  • 製品名Anti-Kir2.1 antibody [S21-32]
    Kir2.1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [S21-32] to Kir2.1
  • 特異性No cross reactivity against Kir2.2, or Kir2.3
  • アプリケーション適用あり: WB, ICC, ICC/IF, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Fusion protein, corresponding to amino acids 41-64 and 189-428 of mouse Kir2.1 (NP_032451).

  • ポジティブ・コントロール
    • Rat brain lysate. IF/ICC: SKNSH cell line.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名S21-32
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab85492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. If bands are weak, use lysate without boiling, heat at 37°C for 15 minutes.
ICC Use a concentration of 0.1 - 1 µg/ml. (Perox)
ICC/IF Use a concentration of 1 - 10 µg/ml.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium or cesium.
  • 組織特異性Heart, brain, placenta, lung, skeletal muscle, and kidney. Diffusely distributed throughout the brain.
  • 関連疾患Defects in KCNJ2 are the cause of long QT syndrome type 7 (LQT7) [MIM:170390]; also called Andersen syndrome or Andersen cardiodysrhythmic periodic paralysis. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. LQT7 manifests itself as a clinical triad consisting of potassium-sensitive periodic paralysis, ventricular ectopy and dysmorphic features.
    Defects in KCNJ2 are the cause of short QT syndrome type 3 (SQT3) [MIM:609622]. Short QT syndromes are heart disorders characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death. SQT3 has a unique ECG phenotype characterized by asymmetrical T waves.
  • 配列類似性Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ2 subfamily.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cardiac inward rectifier potassium channel antibody
    • HHBIRK 1 antibody
    • HHBIRK1 antibody
    • HHIRK 1 antibody
    • HHIRK1 antibody
    • HIRK 1 antibody
    • hIRK1 antibody
    • Inward rectifier K antibody
    • Inward rectifier K(+) channel Kir2.1 antibody
    • Inward rectifier potassium channel 2 antibody
    • inwardly rectifying subfamily J member 2 antibody
    • IRK 1 antibody
    • IRK-1 antibody
    • IRK1 antibody
    • IRK2_HUMAN antibody
    • KCNJ2 antibody
    • KIR2.1 antibody
    • LQT 7 antibody
    • LQT7 antibody
    • Potassium channel antibody
    • Potassium channel inwardly rectifying subfamily J member 2 antibody
    • Potassium inwardly rectifying channel J2 antibody
    • Potassium inwardly rectifying channel subfamily J member 2 antibody
    • SQT 3 antibody
    • SQT3 antibody
    see all

Anti-Kir2.1 antibody [S21-32] 画像

  • ICC/IF image of ab85492 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (abX85492, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab85492 staining Kir2.1 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).
  • Overlay histogram showing SH-SY5Y cells stained with ab85492 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85492, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Kir2.1 antibody [S21-32] (ab85492) 使用論文

This product has been referenced in:
  • Goldoni D  et al. A novel dual-fluorescence strategy for functionally validating microRNA targets in 3-prime untranslated regions: regulation of the inward rectifier potassium channel Kir2.1 by miR-212. Biochem J : (2012). WB ; Human . Read more (PubMed: 22880819) »

See 1 Publication for this product

Product Wall

Thank you for contacting us.
The immunogen of ab80969 shares 85% homology with Zebrafish so it is more likely to cross-react. Once you decide to buy the antibody I would like to inform you about the Abreview 100% discount programme. Please click t...

Read More

Thank youand your customer very much for your interest in our antibodies.

I checked all immunogens and homology to zebrafish (sequnec that your customer provided)of the Kir2.1 antibodies we offer at the moment.

Only one antibody sho...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"