製品の概要

  • 製品名Anti-Kinetochore antibody
    Kinetochore 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Kinetochore
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Xenopus tropicalis , Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide, corresponding to a region within residues 375 and 425 of human Kinetochore (NP_055523.1).

  • ポジティブ・コントロール
    • HeLa whole cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use at 2-5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 250 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性The kinetochore is the protein structure in eukaryotes which assembles on the centromere and links the chromosome to microtubule polymers from the mitotic spindle during mitosis and meiosis. The kinetochore contains two regions: an inner kinetochore, which is tightly associated with the centromere DNA; and an outer kinetochore, which interacts with microtubules. Even the simplest kinetochores consist of more than 45 different proteins, many of which are conserved throughout eukaryote species.
    • 細胞内局在Cytoplasmic and Nuclear
    • 参照データベース
    • 別名
      • HsROD antibody
      • Kinetochore associated 1 antibody
      • KNTC1 antibody
      • ROD antibody
      • Rough Deal homolog, centromere/kinetochore protein antibody
      see all

    Anti-Kinetochore antibody 画像

    • All lanes : Anti-Kinetochore antibody (ab85996) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 250 kDa
      Observed band size : 250 kDa
      Additional bands at : 115 kDa,175 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 10 seconds
    • Detection of Kinetochore by Western Blot of Immunprecipitate.
      ab85996, at 1 µg/ml, staining Kinetochore in HeLa whole cell lysate immunoprecipitated using ab8599 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 10 seconds.

    Anti-Kinetochore antibody (ab85996) 使用論文

    ab85996 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"