製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Kinesin 5A
  • アプリケーション
    適用あり: WB, IHC-P, ICCmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to 20 internal sequence amino acids of Human Kinesin 5A (NP_004975.2).

  • ポジティブ・コントロール
    • Human Brain, cortex tissue; K562 cell lysate; K562 cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab118534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 117 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL).
  • 組織特異性
    Distributed throughout the CNS but is highly enriched in subsets of neurons.
  • 関連疾患
    Defects in KIF5A are the cause of spastic paraplegia autosomal dominant type 10 (SPG10) [MIM:604187]. An inherited degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity (stiffness) of the legs. Rate of progression and the severity of symptoms is quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.
  • 配列類似性
    Belongs to the kinesin-like protein family. Kinesin subfamily.
    Contains 1 kinesin-motor domain.
  • ドメイン
    Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
  • 細胞内局在
    Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton. Concentrated in the cell body of the neurons, particularly in the perinuclear region.
  • Information by UniProt
  • 参照データベース
  • 別名
    • D12S1889 antibody
    • KIF 5A antibody
    • Kif5a antibody
    • KIF5A_HUMAN antibody
    • Kinesin family member 5A antibody
    • Kinesin heavy chain isoform 5A antibody
    • Kinesin Heavy Chain Neuron Specific antibody
    • Kinesin heavy chain neuron-specific 1 antibody
    • MY050 antibody
    • Neuronal kinesin heavy chain antibody
    • NKHC 1 antibody
    • NKHC antibody
    • SPG 10 antibody
    see all

画像

  • Human Brain, Cortex tissue (formalin-fixed, paraffin-embedded) stained with ab118534 at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Lane 1 : Anti-Kinesin 5A antibody (ab118534) at 0.5 µg/ml
    Lane 2 : Anti-Kinesin 5A antibody (ab118534) at 1 µg/ml

    Lane 1 : K562 cell lysate
    Lane 2 : K562 cell lysate


    Predicted band size : 117 kDa
  • Immunocytochemical analysis of Kinesin 5A expression in K562 cells using ab118534 at 5 µg/ml.

参考文献

This product has been referenced in:

See 1 Publication for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab118534.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録